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ISRN Analytical Chemistry
Volume 2013 (2013), Article ID 948547, 5 pages
Research Article

RP-HPLC Method Development and Validation for Simultaneous Estimation of Clarithromycin and Paracetamol

Department of Quality Assurance, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara-Punjab 144411, India

Received 11 June 2013; Accepted 21 July 2013

Academic Editors: B. J. Birch, C. M. Chan, R. N. Rao, and B. Rittich

Copyright © 2013 Sadana Gangishetty and Surajpal Verma. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present work describes a simple, rapid, and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of clarithromycin (CLA) and paracetamol (PCM). C18 column (Kromasil ODS, 5 µm, 250 × 4.6 mm) and a mobile phase containing phosphate buffer (0.05 M) along with 1-octane sulphonic acid sodium salt monohydrate (0.005 M) adjusted to pH 3.2: acetonitrile (50 : 50 v/v) mixture was used for the separation and quantification. The flow rate was 1.0 mL/min and the eluents were detected by UV detector at 205 nm. The retention times were found to be 2.21 and 3.73 mins, respectively. The developed method was validated according to ICH guidelines Q2 (R1) and found to be linear within the range of 75–175 µg/mL for both drugs. The developed method was applied successfully for assay of clarithromycin and paracetamol in their combined in-house developed dosage forms and in vitro dissolution studies.