Extrahepatic 25-Hydroxylation of Vitamin D3 in an Engineered Osteoblast Precursor Cell Line Exploring the Influence on Cellular Proliferation and Matrix Maturation during Bone Development
Figure 6
Osteoblast precursor cell lines OPC1 stained with ARS after two weeks in experimental conditions. Images of OPC1 cells without either osteogenic factors (BM−) or vitamin D (ethanol control) (a), BM− with 1 μM vitamin D3 (b), and BM− with 10 μM vitamin D3 (c) consistently did not absorb a statistically perceptible amount of ARS compared to groups containing osteogenic factors (BM+) (d–f). However, cells cultured with BM+ and 1 μM vitamin D3 (e) and BM+ with 10 μM vitamin D3 (f) had a statistically significant () amount of calcium associated with ECM mineralization compared to the BM+ control (d). Magnification = ×10, scale = 500 μm.
