Extrahepatic 25-Hydroxylation of Vitamin D3 in an Engineered Osteoblast Precursor Cell Line Exploring the Influence on Cellular Proliferation and Matrix Maturation during Bone Development
Figure 7
Osteoblast precursor cell line MC3T3-E1 stained with ARS after two weeks within the experimental treatment groups. MC3T3-E1 cells without either osteogenic factors (BM−) or vitamin D (ethanol control) (a), BM− with 1 μM vitamin D3 (b), and BM− with 10 nM 1,25OH2D3 (c) consistently did not absorb a significant amount of ARS compared to groups containing osteogenic factors (BM+) (d–f). Cells cultured with vitamin D3 and BM+ (e) were statistically significant compared to the negative control (a) but were not comparable with the parallel control (d). However, cells cultured with BM+ and 10 nM 1,25OH2D3 (f) had a statistically significant () amount of calcium associated with ECM mineralization compared to the BM+ control (d). Magnification = ×10, scale = 500 μm.
