Better Rooting Procedure to Enhance Survival Rate of Field Grown Malaysian Eksotika Papaya Transformed with 1-Aminocyclopropane-1-Carboxylic Acid Oxidase Gene
Figure 2
PCR analysis of transformed, regenerated shoots. (a) PCR analysis of genomic DNA obtained from putative transformants, harbouring the RNAi constructs, using gusA and PDK intron primers amplifying 1711 and 622 bp products, respectively. L1–L3 are putativetransgenic shoots, and (b) PCR analysis of antisense putative transgenic papaya plants using nptII primers producing a 610 bp product. L1–L10 are putative transgenic shoots. M1 and M2 represent 1 kb and 100 bp DNA ladders, respectively (Biolabs, California, USA), PO is the pOpOff 2 vector, and P is the pASACO2E1 plasmid as a positive control.
