Better Rooting Procedure to Enhance Survival Rate of Field Grown Malaysian Eksotika Papaya Transformed with 1-Aminocyclopropane-1-Carboxylic Acid Oxidase Gene

Table 3

Primers used for analysis of antisense and RNAi constructs in transformed papaya shoots.

Name of construct	Primer code	Primer sequence (5′-3′)	Length (bp)	Amplicon size (bp)
	nptIIF	ccttatccgcaacttctttacc	22	610
Antisense	nptIIR	caccatgatattcggcaagcag	22
(pASACO2E1)	643F	actgacgtaagggatga	17	1700
	643R	tacattgccgtagatga	17
	nptIIF	ccttatccgcaacttctttacc	22	610
	nptIIR	caccatgatattcggcaagcag	22
	gusAF	catggtacgtcctgtagaaacc	22	1711
	gusAR	gaagatccctttcttgttaccg	22
RNAi	PDKF	ttcccaactgtaatcaatcc	20	622
pRNAiACO1	PDKR	tgacaagtgatgtgtaagac	20
pRNAiACO2	pRNAiACO1F	cttctttctacaaccccagc	20	350
pRNAiCACO	pRNAiACO1R	gacaccgttttcccacact	19
	pRNAiACO2F	tctactgtaactcctggtgc	20	317
	pRNAiACO2R	caccaaaggccactagacag	20
	pRNAiCACOF	atgaaggagtttgcagtggg	20	340
	pRNAiCACOR	ccgttagtaatcagctcaag	20