Research Article
Enhanced Cellulase Production from Bacillus subtilis by Optimizing Physical Parameters for Bioethanol Production
Table 5
Bioethanol production by SSF from wild grass employing B. subtilis cellulase and Z. mobilis.
| Pretreatment | Substrate concentration (%, w/v) | Mode of SSF | SSF process parameters | Reducing sugar* (g/L) | Ethanol yield (g/g) | Ethanol titre* (g/L) |
| Acid acetone | 1% | Shake flask | 1.10 ± 0.07 | 0.093 | 0.93 ± 0.07 | Alkali | 1% | Shake flask | 1.25 ± 0.04 | 0.098 | 0.98 ± 0.06 | Alkali | 1% | Bioreactor | 2.51 ± 0.03 | 0.22 | 2.23 ± 0.08 | Alkali | 5% | Shake flask | 9.08 ± 0.06 | 0.15 | 7.56 ± 0.05 | Alkali | 5% | Bioreactor | 13.22 ± 0.04 | 0.23 | 11.65 ± 0.04 |
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values correspond to the maximum reducing sugar and maximum ethanol titre at a particular time. Values are mean ± SE ().
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