Comparison of Lipase Production by Enterococcus faecium MTCC 5695 and Pediococcus acidilactici MTCC 11361 Using Fish Waste as Substrate: Optimization of Culture Conditions by Response Surface Methodology

Table 4

Actual levels of independent variables with the observed values of the response variable, Lipase activity (𝑌₁ of MTCC 5695, 𝑌₂ of PA-63).

Run #	𝑋1	𝑋2	𝑋3	Y 1	Y 2
1	1	0.1	48	598	10.48
2	5	0.1	48	1128	255.47
3	1	0.2	48	452	49.60
4	5	0.2	48	1160	293.61
5	1	0.15	24	437	214.96
6	5	0.15	24	1707	487.22
7	1	0.15	72	654	55.81
8	5	0.15	72	977	387.92
9	3	0.1	24	813	294.92
10	3	0.2	24	799	346.98
11	3	0.1	72	567	279.61
12	3	0.2	72	667	434.99
13	3	0.15	48	820	394.92
14	3	0.15	48	829	396.32
15	3	0.15	48	913	396.58

𝑋1: FWO concentration, % v/v; 𝑋2: FWPH concentration, mg/mL; 𝑋3: time, hours.
𝑌₁: lipase activity (U/mL) of MTCC 5695; 𝑌₂: lipase activity of MTCC 11361.