Table of Contents
ISRN Genomics
Volume 2013 (2013), Article ID 980651, 7 pages
http://dx.doi.org/10.1155/2013/980651
Research Article

Gene Expression Analysis of the IPEC-J2 Cell Line: A Simple Model for the Inflammation-Sensitive Preterm Intestine

1Innate Immunology Group, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1870 Frederiksberg C, Denmark
2Department of Nutrition, Exercise and Sports, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark

Received 31 January 2013; Accepted 20 February 2013

Academic Editors: H. Fuchs and P. Wigley

Copyright © 2013 Ann Cathrine F. Støy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. S. Pitman and R. S. Blumberg, “First line of defense: the role of the intestinal epithelium as an active component of the mucosal immune system,” Journal of Gastroenterology, vol. 35, no. 11, pp. 805–814, 2000. View at Publisher · View at Google Scholar · View at Scopus
  2. P. Schierack, M. Nordhoff, M. Pollmann et al., “Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine,” Histochemistry and Cell Biology, vol. 125, no. 3, pp. 293–305, 2006. View at Publisher · View at Google Scholar · View at Scopus
  3. E. J. A. Veldhuizen, I. Koomen, T. Ultee, A. van Dijk, and H. P. Haagsman, “Salmonella serovar specific upregulation of porcine defensins 1 and 2 in a jejunal epithelial cell line,” Veterinary Microbiology, vol. 136, no. 1-2, pp. 69–75, 2009. View at Publisher · View at Google Scholar · View at Scopus
  4. V. Mariani, S. Palermo, S. Fiorentini, A. Lanubile, and E. Giuffra, “Gene expression study of two widely used pig intestinal epithelial cell lines: IPEC-J2 and IPI-2I,” Veterinary Immunology and Immunopathology, vol. 131, no. 3-4, pp. 278–284, 2009. View at Publisher · View at Google Scholar · View at Scopus
  5. T. E. Burkey, K. A. Skjolaas, S. S. Dritz, and J. E. Minton, “Expression of porcine Toll-like receptor 2, 4 and 9 gene transcripts in the presence of lipopolysaccharide and Salmonella enterica serovars Typhimurium and Choleraesuis,” Veterinary Immunology and Immunopathology, vol. 130, no. 1-2, pp. 96–101, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. M. M. Geens and T. A. Niewold, “Preliminary characterization of the transcriptional response of the porcine intestinal cell line IPEC-J2 to enterotoxigenic escherichia coli, escherichia coli, and E. coli lipopolysaccharide,” Comparative and Functional Genomics, vol. 2010, Article ID 469583, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. L. D. Schmidt, L. J. Kohrt, and D. R. Brown, “Comparison of growth phase on Salmonella enterica serovar Typhimurium invasion in an epithelial cell line (IPEC J2) and mucosal explants from porcine small intestine,” Comparative Immunology, Microbiology and Infectious Diseases, vol. 31, no. 1, pp. 63–69, 2008. View at Publisher · View at Google Scholar · View at Scopus
  8. A. K. Sonntag, M. Bielaszewska, A. Mellmann et al., “Shiga toxin 2e-producing Escherichia coli isolates from humans and pigs differ in their virulence profiles and interactions with intestinal epithelial cells,” Applied and Environmental Microbiology, vol. 71, no. 12, pp. 8855–8863, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. P. W. Lin and B. J. Stoll, “Necrotising enterocolitis,” The Lancet, vol. 368, no. 9543, pp. 1271–1283, 2006. View at Publisher · View at Google Scholar · View at Scopus
  10. M. A. Quigley, G. Henderson, M. Y. Anthony, and W. McGuire, “Formula milk versus donor breast milk for feeding preterm or low birth weight infants,” Cochrane Database of Systematic Reviews, no. 4, Article ID CD002971, 2007. View at Google Scholar · View at Scopus
  11. C. R. Bjornvad, T. Thymann, N. E. Deutz et al., “Enteral feeding induces diet-dependent mucosal dysfunction, bacterial proliferation, and necrotizing enterocolitis in preterm pigs on parenteral nutrition,” American Journal of Physiology, vol. 295, no. 5, pp. G1092–G1103, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. P. T. Sangild, R. H. Siggers, M. Schmidt et al., “Diet- and colonization-dependent intestinal dysfunction predisposes to necrotizing enterocolitis in preterm pigs,” Gastroenterology, vol. 130, no. 6, pp. 1776–1792, 2006. View at Publisher · View at Google Scholar · View at Scopus
  13. T. Thymann, D. G. Burrin, K. A. Tappenden, C. R. Bjornvad, S. K. Jensen, and P. T. Sangild, “Formula-feeding reduces lactose digestive capacity in neonatal pigs,” British Journal of Nutrition, vol. 95, no. 6, pp. 1075–1081, 2006. View at Publisher · View at Google Scholar · View at Scopus
  14. E. R. Van Haver, P. T. Sangild, M. Oste, J. L. A. Siggers, A. L. M. Weyns, and C. J. Van Ginneken, “Diet-dependent mucosal colonization and interleukin-1β responses in preterm pigs susceptible to necrotizing enterocolitis,” Journal of Pediatric Gastroenterology and Nutrition, vol. 49, no. 1, pp. 90–98, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. R. J. Playford, C. E. Macdonald, and W. S. Johnson, “Colostrum and milk-derived peptide growth factors for the treatment of gastrointestinal disorders,” American Journal of Clinical Nutrition, vol. 72, no. 1, pp. 5–14, 2000. View at Google Scholar · View at Scopus
  16. R. H. Siggers, T. Thymann, B. B. Jensen et al., “Elective cesarean delivery affects gut maturation and delays microbial colonization but does not increase necrotizing enterocolitis in preterm pigs,” American Journal of Physiology, vol. 294, no. 3, pp. R929–R938, 2008. View at Publisher · View at Google Scholar · View at Scopus
  17. K. Skovgaard, S. Mortensen, M. Boye, J. Hedegaard, and P. M. H. Heegaard, “Hepatic gene expression changes in pigs experimentally infected with the lung pathogen Actinobacillus pleuropneumoniae as analysed with an innate immunity focused microarray,” Innate Immunity, vol. 16, no. 6, pp. 343–353, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. M. S. Cilieborg, K. Skovgaard, L. M. Nørgaard, P. T. Sangild, and M. Boye, “Intestinal colonization, gut function and inflammatory responses are moderately influenced by gestational age at birth,” in Proceedings of the 14th International Symposium on Microbial Ecology, 2012.
  19. J. Vandesompele, K. De Preter, F. Pattyn et al., “Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes,” Genome Biology, vol. 3, no. 7, Article ID RESEARCH0034, 2002. View at Google Scholar · View at Scopus
  20. C. L. Andersen, J. L. Jensen, and T. F. Ørntoft, “Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets,” Cancer Research, vol. 64, no. 15, pp. 5245–5250, 2004. View at Publisher · View at Google Scholar · View at Scopus
  21. H. R. Sargeant, H. M. Miller, and M. A. Shaw, “Inflammatory response of porcine epithelial IPEC J2 cells to enterotoxigenic E. coli infection is modulated by zinc supplementation,” Molecular Immunology, vol. 48, pp. 2113–2121, 2011. View at Google Scholar
  22. R. I. Lehrer, “Primate defensins,” Nature Reviews Microbiology, vol. 2, no. 9, pp. 727–738, 2004. View at Publisher · View at Google Scholar · View at Scopus
  23. A. M. E. Sidahmed, A. J. León, S. E. Bosinger et al., “CXCL10 contributes to p38-mediated apoptosis in primary T lymphocytes in vitro,” Cytokine, vol. 59, no. 2, pp. 433–441, 2012. View at Google Scholar
  24. K. Skovgaard, S. Cirera, D. Vasby et al., “Expression of innate immune genes, proteins and microRNAs in lung tissue of pigs infected experimentally with influenza virus (H1N2),” Innate Immunity, 2013. View at Publisher · View at Google Scholar