An Immunological Approach to Increase the Brain’s Resilience to Insults
Figure 1
Generation of the NR1 fragments and screening of resultant vaccine antisera. (a) Schematic representation of the NMDAR1 subunit and (b) the engineered and generated NR1 fragments (figure adapted from http://www.pharmacology2000.com/Central/Opioids/Advanced_opioids3.htm). (c) SDS-PAGE analysis of washed inclusion bodies from E. coli expressing each of the NR1 fragments, showing degree of purity and molecular weight prior to vaccination. (d) Protein-G purified IgG from NR1 preimmune or immune rat sera was used at 100 mg/mL on naïve hippocampal sections. The affinity-purified commercial NR1 polyclonal antibody (Chemicon AB1516) was used at 1 : 200. Scale bar = 400 μm.
