An Immunological Approach to Increase the Brain’s Resilience to Insults

Table 1

(a) Mean antibody titers. (b) Specificity of vaccine sera for native NR1.

(a)

	Vaccination group
	NR1[21–375]	NR1[313–619]	NR1[654–800]	Homer1a
Titer at week 7; (𝑛=20)	10082±4884	10670±4405	23457±8300	27174±14572
Titer at week 18; (𝑛=6)	5370±1377	5712±1471	8891±1836	11586±2886

Sera were collected 7 and 18 weeks after vaccination and antigen-specific titers were determined. Data are presented as mean ± SD.

(b)

Vaccination group	Rat ID	Antigen-specific titer	OD at 450 nm
			1 : 90	1 : 810
NR1[21–375]	H6	17556	0.432 (0.069)	0.185 (0.066)
	H3	8982	0.336 (0.066)	0.163 (0.069)
NR1[313–619]	K2	23879	0.491 (0.069)	0.484 (0.074)
	K19	13194	0.461 (0.079)	0.345 (0.058)
NR1[654–800]	J3	39149	0.516 (0.061)	0.364 (0.058)
	J15	23879	0.376 (0.057)	0.243 (0.065)
Homer1a	L6	41360	0.082 (0.062)	0.070 (0.062)
	L20	3729	0.068 (0.062)	0.066 (0.063)
AB1516			0.420	0.199

OD at 450 nm represents antibodies bound to immobilised NR1 protein in an antigen capture ELISA (see Section 2). OD at 450 nm for preimmune sera is shown in brackets.