The Identification and Differentiation between Burkholderia mallei and Burkholderia pseudomallei Using One Gene Pyrosequencing

Figure 1

(a) Schematic of Burkholderia mallei fliP gene with IS407A interruption that caused both gene inversion and loss of function. The large arrow within the block designates the orientation of the two parts of the fliP gene on the DNA strand. The location and direction of the various primers are noted by the small arrows. The pyrosequencing target in B. mallei is the interface between the insertion sequence element and fliP gene. (b) Schematic of the Burkholderia pseudomallei intact and functional fliP gene. The large arrow within the block designates the orientation of the intact gene while the location and directions of the primers used are denoted by the small arrows. The pyrosequencing target in B. pseudomallei covers both sides of the point of the fliP gene that corresponds to where the insertion sequence is found in B. mallei.