Table of Contents
International Scholarly Research Notices
Volume 2014 (2014), Article ID 109583, 10 pages
http://dx.doi.org/10.1155/2014/109583
Research Article

The Identification and Differentiation between Burkholderia mallei and Burkholderia pseudomallei Using One Gene Pyrosequencing

Center for Biological Defense, College of Public Health, University of South Florida, Tampa, FL 33612, USA

Received 2 April 2014; Revised 11 July 2014; Accepted 15 July 2014; Published 2 October 2014

Academic Editor: Dennis A. Bazylinski

Copyright © 2014 Damian H. Gilling et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. I. Janse, R. A. Hamidjaja, A. C. A. Hendriks, and B. J. van Rotterdam, “Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei,” BMC Infectious Diseases, vol. 13, no. 1, article 86, 2013. View at Publisher · View at Google Scholar · View at Scopus
  2. S. J. Peacock, H. P. Schweizer, D. A. B. Dance et al., “Management of accidental laboratory exposure to Burkholderia pseudomallei and B. mallei,” Emerging Infectious Diseases, vol. 14, no. 7, article e2, 2008. View at Publisher · View at Google Scholar · View at Scopus
  3. E. B. Silva and S. W. Dow, “Development of Burkholderia mallei and pseudomallei vaccines,” Frontiers in Cellular and Infection Microbiology, vol. 3, p. 10, 2013. View at Publisher · View at Google Scholar · View at Scopus
  4. G. C. Whitlock, D. M. Estes, and A. G. Torres, “Glanders: off to the races with Burkholderia mallei,” FEMS Microbiology Letters, vol. 277, no. 2, pp. 115–122, 2007. View at Publisher · View at Google Scholar · View at Scopus
  5. M. Wheelis, “First shots fired in biological warfare,” Nature, vol. 395, article 213, 1998. View at Publisher · View at Google Scholar · View at Scopus
  6. L. D. Rotz, A. S. Khan, S. R. Lillibridge, S. M. Ostroff, and J. M. Hughes, “Public health assessment of potential biological terrorism agents,” Emerging Infectious Diseases, vol. 8, no. 2, pp. 225–230, 2002. View at Publisher · View at Google Scholar · View at Scopus
  7. B. J. Currie, D. A. B. Dance, and A. C. Cheng, “The global distribution of Burkholderia pseudomallei and melioidosis: an update,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 102, no. 1, pp. S1–S4, 2008. View at Publisher · View at Google Scholar · View at Scopus
  8. V. Ngauy, Y. Lemeshev, L. Sadkowski, and G. Crawford, “Cutaneous melioidosis in a man who was taken as a prisoner of war by the Japanese during World War II,” Journal of Clinical Microbiology, vol. 43, no. 2, pp. 970–972, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. K. C. Kronmann, A. A. Truett, B. R. Hale, and N. F. Crum-Cianflone, “Melioidosis after brief exposure: a serologic survey in US marines,” The American Journal of Tropical Medicine and Hygiene, vol. 80, no. 2, pp. 182–184, 2009. View at Google Scholar · View at Scopus
  10. Centers for Disease Control and Prevention, “Imported melioidosis—south Florida, 2005,” Morbidity and Mortality Weekly Report (MMWR), vol. 55, no. 32, pp. 873–876, 2006. View at Google Scholar
  11. S. E. Dorman, V. J. Gill, J. I. Gallin, and S. M. Holland, “Burkholderia pseudomallei infection in a Puerto Rican patient with chronic granulomatous disease: case report and review of occurrences in the Americas,” Clinical Infectious Diseases, vol. 26, no. 4, pp. 889–894, 1998. View at Publisher · View at Google Scholar · View at Scopus
  12. B. Christenson, Z. Fuxench, J. A. Morales, R. A. Suárez-Villamil, and L. M. Souchet, “Severe community-acquired pneumonia and sepsis caused by Burkholderia pseudomallei associated with flooding in Puerto Rico,” Boletin de la Asociacion Medica de Puerto Rico, vol. 95, no. 6, pp. 17–20, 2003. View at Google Scholar · View at Scopus
  13. D. M. Corral, A. L. Coates, Y. C. W. Yau et al., “Burkholderia pseudomallei infection in a cystic fibrosis patient from the Caribbean: a case report,” Canadian Respiratory Journal, vol. 15, no. 5, pp. 237–239, 2008. View at Google Scholar · View at Scopus
  14. D. M. Engelthaler, J. Bowers, J. A. Schupp et al., “Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA,” PLoS Neglected Tropical Diseases, vol. 5, no. 10, Article ID e1347, 2011. View at Publisher · View at Google Scholar · View at Scopus
  15. D. B. Rolim, M. F. G. Rocha, R. S. N. Brilhante et al., “Environmental isolates of Burkholderia pseudomallei in Ceara State, Northeastern Brazil,” Applied and Environmental Microbiology, vol. 75, no. 4, p. 1215, 2009. View at Publisher · View at Google Scholar
  16. Centers for Disease Control and Prevention, “Laboratory exposure to Burkholderia pseudomallei—Los Angeles, California, 2003,” MMWR Morbidity and Mortality Weekly Report, vol. 53, no. 42, pp. 988–990, 2004. View at Google Scholar
  17. K. Vidyalakshmi, M. Chakrapani, B. Shrikala, S. Damodar, S. Lipika, and S. Vishal, “Tuberculosis mimicked by melioidosis,” International Journal of Tuberculosis and Lung Disease, vol. 12, no. 10, pp. 1209–1215, 2008. View at Google Scholar · View at Scopus
  18. T. J. Inglis and A. Q. Sousa, “The public health implications of melioidosis,” Brazilian Journal of Infectious Diseases, vol. 13, no. 1, pp. 59–66, 2009. View at Google Scholar · View at Scopus
  19. C. Weissert, G. Dollenmaier, P. Rafeiner, J. Riehm, and D. Schultze, “Burkholderia pseudomallei misidentified by automated system,” Emerging Infectious Diseases, vol. 15, no. 11, pp. 1799–1801, 2009. View at Publisher · View at Google Scholar · View at Scopus
  20. S. A. Batra, S. Krupanidhi, and U. Tuteja, “A sensitive & specific multiplex PCR assay for simultaneous detection of Bacillus anthracis, Yersinia pestis, Burkholderia pseudomallei & Brucella species,” Indian Journal of Medical Research, vol. 138, no. 1, pp. 111–116, 2013. View at Google Scholar · View at Scopus
  21. T. J. J. Inglis, D. Chiang, G. S. H. Lee, and L. Chor-Kiang, “Potential misidentification of Burkholderia pseudomallei by API 20NE,” Pathology, vol. 30, no. 1, pp. 62–64, 1998. View at Publisher · View at Google Scholar · View at Scopus
  22. M. B. Glass and T. Popovic, “Preliminary evaluation of the API 20NE and RapID NF plus systems for rapid identification of Burkholderia pseudomallei and B. mallei,” Journal of Clinical Microbiology, vol. 43, no. 1, pp. 479–483, 2005. View at Publisher · View at Google Scholar · View at Scopus
  23. A. J. Brent, P. C. Matthews, D. A. Dance, T. L. Pitt, and R. Handy, “Misdiagnosing melioidosis,” Emerging Infectious Diseases, vol. 13, no. 2, pp. 349–351, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. T. J. J. Inglis, A. Merritt, G. Chidlow, M. Aravena-Roman, and G. Harnett, “Comparison of diagnostic laboratory methods for identification of Burkholderia pseudomallei,” Journal of Clinical Microbiology, vol. 43, no. 5, pp. 2201–2206, 2005. View at Publisher · View at Google Scholar · View at Scopus
  25. W. Lowe, K. J. March, J. A. Bunnell, OL. K. Neill, and A. R. Robison, “PCR-based methodologies used to detect and differentiate the Burkholderia pseudomallei complex,” Current Issues in Intestinal Microbiology, vol. 16, pp. 23–54, 2013. View at Google Scholar
  26. D. Limmathurotsakul, V. Wuthiekanun, P. Amornchai, G. Wongsuwan, N. P. J. Day, and S. J. Peacock, “Effectiveness of a simplified method for isolation of Burkholderia pseudomallei from soil,” Applied and Environmental Microbiology, vol. 78, no. 3, pp. 876–877, 2012. View at Publisher · View at Google Scholar · View at Scopus
  27. J. R. Bowers, D. M. Engelthaler, J. L. Ginther et al., “BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei,” PLoS ONE, vol. 5, no. 11, Article ID e15413, 2010. View at Publisher · View at Google Scholar · View at Scopus
  28. V. A. Antonov, G. A. Tkachenko, V. V. Altukhova et al., “Molecular identification and typing of Burkholderia pseudomallei and Burkholderia mallei: when is enough enough?” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 102, no. 1, pp. S134–S139, 2008. View at Publisher · View at Google Scholar · View at Scopus
  29. W. Ruppitsch, A. Stöger, A. Indra et al., “Suitability of partial 16S ribosomal RNA gene sequence analysis for the identification of dangerous bacterial pathogens,” Journal of Applied Microbiology, vol. 102, no. 3, pp. 852–859, 2007. View at Publisher · View at Google Scholar · View at Scopus
  30. W. G. Weisburg, S. M. Barns, D. A. Pelletier, and D. J. Lane, “16S ribosomal DNA amplification for phylogenetic study,” Journal of Bacteriology, vol. 173, no. 2, pp. 697–703, 1991. View at Google Scholar · View at Scopus
  31. T. G. Lessie, W. Hendrickson, B. D. Manning, and R. Devereux, “Genomic complexity and plasticity of Burkholderia cepacia,” FEMS Microbiology Letters, vol. 144, no. 2-3, pp. 117–128, 1996. View at Publisher · View at Google Scholar · View at Scopus
  32. H. Song, J. Hwang, H. Yi et al., “The early stage of bacterial genome-reductive evolution in the host,” PLoS Pathogens, vol. 6, no. 5, pp. 1–10, 2010. View at Publisher · View at Google Scholar · View at Scopus
  33. W. C. Nierman, D. DeShazer, H. S. Kim et al., “Structural flexibility in the Burkholderia mallei genome,” Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 39, pp. 14246–14251, 2004. View at Publisher · View at Google Scholar · View at Scopus
  34. M. Ronaghi, “Pyrosequencing sheds light on DNA sequencing,” Genome Research, vol. 11, no. 1, pp. 3–11, 2001. View at Publisher · View at Google Scholar · View at Scopus
  35. U.S. Department of Health and Human Services CDC & NIH, Biosafety in Microbiological and Biomedical Laboratories, Washington, DC, USA, 5th edition, 2007.
  36. E. E. Galyov, P. J. Brett, and D. Deshazer, “Molecular insights into Burkholderia pseudomallei and Burkholderia mallei pathogenesis,” Annual Review of Microbiology, vol. 64, pp. 495–517, 2010. View at Publisher · View at Google Scholar · View at Scopus
  37. M. D. Fakruddin, A. Chowdhury, M. D. Hossain, S. K. Bin Mannan, and N. R. Mazurmdar, “Pyrosequencing-principles and applications,” Life Science, vol. 2, pp. 65–76, 2012. View at Google Scholar
  38. B. Gharizadeha, M. Akhrasa, N. Nourizadb et al., “Methodological improvements of pyrosequencing technology,” Journal of Biotechnology, vol. 124, no. 3, pp. 504–511, 2006. View at Google Scholar
  39. J. Malakooti, B. Ely, and P. Matsumura, “Molecular characterization, nucleotide sequence, and expression of the fliO, fliP, fliQ, and fliR genes of Escherichia coli,” Journal of Bacteriology, vol. 176, no. 1, pp. 189–197, 1994. View at Google Scholar · View at Scopus
  40. F. M. Thibault, E. Valade, and D. R. Vidal, “Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes,” Journal of Clinical Microbiology, vol. 42, no. 12, pp. 5871–5874, 2004. View at Publisher · View at Google Scholar · View at Scopus
  41. L. Losada, C. M. Ronningf, D. Deshazer et al., “Continuing evolution of Burkholderia mallei through genome reduction and large-scale rearrangements,” Genome Biology and Evolution, vol. 2, no. 1, pp. 102–116, 2010. View at Publisher · View at Google Scholar · View at Scopus
  42. D. N. Fredricks and D. A. Relman, “Application of polymerase chain reaction to the diagnosis of infectious diseases,” Clinical Infectious Diseases, vol. 29, no. 3, pp. 475–488, 1999. View at Publisher · View at Google Scholar · View at Scopus
  43. S. Yang and R. E. Rothman, “PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings,” The Lancet Infectious Diseases, vol. 4, no. 6, pp. 337–348, 2004. View at Publisher · View at Google Scholar · View at Scopus