The Identification and Differentiation between Burkholderia mallei and Burkholderia pseudomallei Using One Gene Pyrosequencing

Table 1

List of bacteria used in this study.

Organism	CBD number^a	Historical strain identifier
Burkholderia
Burkholderia mallei	BB 0044	China 5 (MM-A, NBL 4) ATCC 10399
Burkholderia mallei	BB 0045	Ivan (NCTC 10230) ATCC 15310
Burkholderia mallei	BB 0046	China 7 (NBL 7) ATCC 23344
Burkholderia mallei	BB 0050	GB8 horse 4
Burkholderia mallei	DD-675	BURK014
Burkholderia mallei	DD-671	BURK005 (SR092700A)
Burkholderia mallei	DD-372	BURK007 (SR092700C)
Burkholderia mallei	DD-673	BURK009 (SR092700E)
Burkholderia mallei	DD-677	BURK062 (Turkey 1)
Burkholderia mallei	DD-178	BURK010 (2344)
Burkholderia pseudomallei	BB 0047	China 3 (MP-H, NBL 104)
Burkholderia pseudomallei	BB 0048	S 397 (NRRL B-1112, CCEB 472)
Burkholderia pseudomallei	BB 0049	286 (MP-S, NBL 121)
Burkholderia pseudomallei	BB 0051	K96243
Burkholderia pseudomallei	BB 0052	1026b
Burkholderia pseudomallei	BB 0053	1106b
Burkholderia pseudomallei	BB 0054	1710a
Burkholderia pseudomallei	BURK088	BURK088
Burkholderia pseudomallei	BURK099	BURK099
Burkholderia pseudomallei	NR-9320	K96423
Burkholderia cepacia	CBD 1341	FL-M-05-0506072
Burkholderia cepacia	CBD 1342	FL-M-05-B24210
Burkholderia cepacia	CBD 1343	FL-M-05-B25995R
Burkholderia cepacia	CBD 1442	ATCC BAA-245
Burkholderia cepacia	CBD 1450	F68492
Burkholderia cepacia	CBD 1456	H27659
Burkholderia cepacia	CBD 1455	H35975
Burkholderia cepacia	CBD 1449	M25311
Burkholderia cepacia	CBD 1458	M27066
Burkholderia cepacia	CBD 1451	M42544
Burkholderia cepacia	CBD 1453	M52455
Burkholderia cepacia	CBD 1454	M74393
Burkholderia cepacia	CBD 1457	T10400
Burkholderia cepacia	CBD 1452	T33589
Burkholderia cepacia	CBD 1448	T47491
Burkholderia graminis	CBD 1440	ATCC 700544
Burkholderia multivorans	CBD 1443	ATCC BAA-247
Burkholderia stabilis	CBD 1441	ATCC BAA-67
Burkholderia vietnamiensis	CBD 1438	ATCC 55792
Burkholderia vietnamiensis	CBD 1444	ATCC BAA-248
Burkholderia thailandensis	CBD 1439	ATCC 700388
Burkholderia thailandensis	BURK254	E254
Burkholderia thailandensis	BURK43	MSMB043
Other bacteria
Achromobacter xylosoxidans	CBD 0307	ATCC 27061
Acinetobacter baumannii	CBD 1323^b	ATCC 19606
Acinetobacter calcoaceticus	CBD 1336^b	ATCC 23055
Bacillus badius	CBD 0091	ATCC 6462
Cedecea neteri	CBD 0309	ATCC 33855
Citrobacter freundii	CBD 0553^b	ATCC 8090
Enterobacter cloacae	CBD 0556^b	ATCC 13047
Enterococcus faecalis	CBD 1406	ATCC 49532
Enterococcus faecalis	CBD 1407	ATCC 49533
Klebsiella pneumoniae	CBD 0555^b	ATCC 35657
Lactobacillus rhanmosus	CBD 1409	ATCC 53103
Pseudomonas aeruginosa	CBD 0551^b	ATCC 15442
Serratia marcescens	CBD 1239	ATCC 13880
Staphylococcus aureus	CBD 0534	WA-HMC-03-4905
Staphylococcus aureus	CBD 1276	ATCC BAA-976
Stenotrophomonas maltophilia	CBD 0552^b	ATCC 51331
Streptococcus pneumoniae	CBD 1405	ATCC 700669

CBD: CBD stands for “Center for Biological Defense” and is the starting designation we use for bacterial strains that are in our BSL2 bacterial collection. Besides the CBD letter designation, each strain in our collection is also given a number to identify it. Each isolate in our BSL3 bacterial collection has a BB letter designation and then assigned a number as the BSL2 strains.
^bdenotes an isolate carries a homolog of the fliP gene.