Zoledronic Acid Elicits Proinflammatory Cytokine Profile in Osteolytic Prostate Cancer Cells

Figure 2

Effect of ZA on apoptotic and autophagy markers. PC3 cells were treated with ZA at indicated concentration (0~25 μM) for 1, 3, and 6 days. After treatment, total RNA was extracted and cDNA was reverse-transcribed from mRNA for real-time PCR of ATG5 (a), Beclin1 (b), and Bax (d). Total protein was extracted from cells to perform western blot. LC3-II antibody was used to determine the level of autophagosome formation (c). Procaspase-3 antibody was used to measure the activation of caspase-3 (e). Data are expressed as mean ± SD. ^∗𝑃<0.05 compared with control.

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