Table of Contents
ISRN Analytical Chemistry
Volume 2014, Article ID 132020, 5 pages
Research Article

Analytical Method Development and Validation of Solifenacin in Pharmaceutical Dosage Forms by RP-HPLC

1Vignan Pharmacy College, Vadlamudi, Guntur, Andhra Pradesh 522213, India
2Jawaharlal Nehru Technological University Anantapur, Andhra Pradesh 515002, India
3Vaagdevi College of Pharmacy, Gurazala, Guntur, Andhra Pradesh 522415, India

Received 4 January 2014; Accepted 12 February 2014; Published 16 April 2014

Academic Editors: J. Esteve-Romero and A. Tsantili-Kakoulidou

Copyright © 2014 Rihana Parveen Shaik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A new, accurate, precise, and robust HPLC method was developed and validated for the determination of solifenacin in tablet dosage form. The chromatographic separation was achieved on an Inertsil ODS 3V C18 (150 mm × 4.6 mm, 5 μm) stationary phase maintained at ambient temperature with a mobile phase combination of monobasic potassium phosphate (pH 3.5) containing 0.1% triethylamine and methanol (gradient mode) at a flow rate of 1.5 mL/min, and the detection was carried out by using UV detector at 220 nm. The performance of the method was validated according to the present ICH guidelines.