Table of Contents
ISRN Veterinary Science
Volume 2014 (2014), Article ID 148597, 5 pages
http://dx.doi.org/10.1155/2014/148597
Research Article

Malignancy Associated MicroRNA Expression Changes in Canine Mammary Cancer of Different Malignancies

1Institute of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Straße 15, 14163 Berlin, Germany
2Molecular Pulmonology, German Center for Lung Research, Philipps Universität Marburg, Hans-Meerwein-Straße 2, 35043 Marburg, Germany

Received 11 February 2014; Accepted 18 March 2014; Published 2 April 2014

Academic Editors: M. H. Kogut and A. Shamay

Copyright © 2014 Marie-Charlotte von Deetzen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

MicroRNA has been suspected to be generally involved in carcinogenesis since their first description. A first study supported this assumption for canine mammary tumors when miRNA expression was compared to normal gland. The present study extends these results by comparing the expression of 16 microRNA (miRNA) and 4 small nucleolar RNA (snoRNA) in tumors of different malignancy, for example, adenomas, nonmetastasizing and metastasizing carcinomas as well as lymph node metastases, with each other and with normal mammary gland. All neoplastic tissues differed in their miR-210 expression levels from normal gland. While metastatic cells differed in their expression of mir-29b, miR-101, mir-125a, miR-143, and miR-145 from primary tumors, the comparison of miRNA expression in primary tumors of different malignancy failed to reveal significant differences except for a significant downregulation of mir-125a in metastasizing carcinomas when compared to adenomas.