Relevant Aspects of Centrifugation Step in the Preparation of Platelet-Rich Plasma

Table 2

Composition of platelets and WBCs in the P-PRP samples after the second spin step (400 ×g and 10 min). According to the hematocrit of the donor, volumes of the upper phase after first spin ranged from 1.0 to 1.4 mL.

	FcPt	Platelet × 10^3/mm^3 (±SD)	WBC × 10^3/mm^3 (±SD)
P-PRP after second spin step
Blood	—	232±28	—
1/2 of volume of PPP removed	3.1±0.3	668±34	2.2±0.4
1/3 of volume of PPP removed	5.2±0.5	1.222±166	3.3±0.4