Research Article
Purification and Characterization of Haloalkaline, Organic Solvent Stable Xylanase from Newly Isolated Halophilic Bacterium-OKH
Table 3
Purification of xylanase isolated from halobacterium-OKH.
| | | Total protein | Total activity | Specific activity | Fold purity | % yield |
| | Crude enzyme | 525.6 | 574.8 | 1.09 | 1 | 100 | | Ammonium sulphate precipitation | 155.8 | 319.2 | 2.04 | 1.87 | 55.5 | | Ion exchange chromatography | 42.3 | 185.9 | 4.39 | 4.02 | 32.3 | | Size exclusion chromatography | 3.5 | 98.5 | 28.14 | 25.81 | 17.1 |
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