Table of Contents
ISRN Dermatology
Volume 2014, Article ID 202876, 6 pages
Research Article

The Effect of Gynostemma pentaphyllum Extract on Mouse Dermal Fibroblasts

Department of Dermatology, Tianjin Medical University General Hospital, International Students’ Building, Tianjin Medical University, Qixiangtai Road, No. 22, Heping District, Tianjin 300070, China

Received 27 November 2013; Accepted 15 January 2014; Published 4 March 2014

Academic Editors: C. Feliciani and Y. Tuzun

Copyright © 2014 Sara Nadia Lobo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. The objective of this paper is to demonstrate the effect of Gynostemma pentaphyllum extract on mouse dermal fibroblasts. Recent studies have shown that this plant may possess great antioxidant properties, which can be very beneficial in combating oxidative stress. Methods. Gynostemma pentaphyllum extract was prepared and mouse dermal fibroblasts were obtained and cultured as per our laboratory protocols. Twelve samples of cells were cultured under the same conditions and both negative and positive controls were established. Induction of oxidative stress was carried out using ultraviolet C (UVC) light. Viable cell count was carried out, using microscopy. The analysis of the overall results was processed using SPSS version 16.0. Results. Statistical analysis showed strong positive correlation between the concentration of Gynostemma pentaphyllum and the mean duration of cell viability (rs = 1), with a high level of statistical significance ( ). Likewise, strong positive correlation existed between trials of cell viability (rs = 0.988–1), with statistical significance ( ). Conclusion. Gynostemma pentaphyllum extract prolongs viability of mouse dermal fibroblasts damaged by UVC light-induced oxidative stress. The results show the potential benefits of this extract on dermal cell aging.