Table of Contents
International Scholarly Research Notices
Volume 2014, Article ID 282971, 5 pages
http://dx.doi.org/10.1155/2014/282971
Research Article

Next Generation Sequencing Analysis of Biofilms from Three Dogs with Postoperative Surgical Site Infection

1Department of Veterinary Pathology, College of Veterinary Medicine, Freie Universitaet Berlin, 14163 Berlin, Germany
2Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Greifswald, 17498 Insel Riems, Germany

Received 5 August 2014; Accepted 21 September 2014; Published 10 December 2014

Academic Editor: Abdul Hamood

Copyright © 2014 L. M. König et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The composition of biofilms in chronic wound infections of dogs is unclear. In the present study, histologically identified biofilms attached to sutures in chronically infected wounds of three dogs were examined by next generation sequencing of total DNA extracted from formalin-fixed and paraffin-embedded tissue samples. The analysis identified an inhomogeneous bacterial composition in three tissues containing biofilms. Some of the identified bacterial families such as Staphylococci and Streptococci have been found before in biofilms associated with human and canine wounds but in this study were quantitatively in the minority. The majority of the reads classified as bacterial sequences had the highest identity with sequences belonging to the Porphyromonadaceae, Deinococcaceae, Methylococcaceae, Nocardiaceae, Alteromonadaceae, and Propionibacteriaceae and thus taxons of so far minor relevance in veterinary medicine.