New Lipase for Biodiesel Production: Partial Purification and Characterization of LipSB 25-4

Table 1

Summary of LipSB 25-4 purification.

Purification step	Total protein (mg)	Total activity (units)	Specific activity (U/mg)	Yield (%)	Fold purification
Crude extract	90.50	3057.47	33.78	100	1
Ammonium sulfate precipitation + dialysis	1.80	149.78	83.21	4.90	2.47
Gel filtration Chromatography	0.1764	16.25	92.12	0.53	2.73