International Scholarly Research Notices

Research Article

New Lipase for Biodiesel Production: Partial Purification and Characterization of LipSB 25-4

Table 2

Effects of different metal ions, enzyme inhibitors, and organic solvents on LipSB 25-4 activity.


Reagent	Residual activity (%)

Control	100
Metal ions
ZnCl₂	114.3
CoCl₂	74.6
NiCl₂	76.2
NaCl	54.0
MnCl₂	19.0
MgCl₂	0.0
CaCl₂	41.3
CdCl₂	57.1
CuCl₂	60.3
Enzyme inhibitors
EDTA	94
β-Mercaptoethanol	108
PMSF	81
Iodoacetic acid	88
SDS	80
Control	100
Organic solvents
Methanol	100.4
Ethanol	99.6
Isopropanol	91.1
Acetone	108.4
Acetonitrile	102.2
DMSO	96.9
Butanol	97.7
Hexane	99.5
n-Heptane	107.0
Isooctane	114.4
Chloroform	101.9
Ethyl acetate	108.4

Lipase preparation was incubated in the presence of 5 mM metal ions, 0.1% enzyme inhibitors, and 50% (v/v) organic solvents for 1 h at 30°C. Residual activity was measured using a standard method with p-NPP and the activity of enzyme without added metal ions, enzyme inhibitors, and organic solvent was taken as 100%.