Table of Contents
International Scholarly Research Notices
Volume 2014 (2014), Article ID 314502, 5 pages
http://dx.doi.org/10.1155/2014/314502
Research Article

Study of Protoscolicidal Effects of Hypertonic Glucose on Protoscolices of Hydatid Cyst at Different Concentrations and Exposure Times

1Department of Parasitology, Qazvin University of Medical Sciences, Qazvin, Iran
2Cellular & Molecular Research Institute, Qazvin University of Medical Sciences, Qazvin, Iran
3Department of Immunology, Qazvin University of Medical Sciences, Qazvin, Iran
4Department of Social Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
5Zanjan University of Medical Sciences, Zanjan, Iran
6Department of Pediatrics, Ghods Hospital, Qazvin University of Medical Sciences, Qazvin, Iran

Received 21 April 2014; Revised 13 September 2014; Accepted 15 September 2014; Published 30 October 2014

Academic Editor: Atilla Arslan

Copyright © 2014 Mojtaba Shahnazi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Surgical operation is the treatment of choice for hydatid cyst. To date, many protoscolicidal substances have been used for inactivation of hydatid cyst contents but most of these compounds may produce several side effects. The aim of this study was to evaluate the protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst at different concentrations and exposure times. Protoscolices were obtained aseptically from the livers of slaughtered sheep at Qazvin abattoir, Iran. Protoscolices were exposed to different concentrations of hypertonic glucose (10%, 15%, 20%, 25%, 30%, 40%, and 50%) at different times (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, and 60 min). Viability of protoscolices was evaluated by 0.1% eosin and the movement of protoscolices flame cells. The highest protoscolicidal effect (100%) of hypertonic glucose was obtained at concentrations 40% and 50% following 40 and 20 min exposure times, respectively. Some protoscolicidal agents show a variety of dangerous complications such as biliary tract fibrosis and liver necrosis; however, hypertonic glucose especially at a concentration of 40% may demonstrate less side effects compared with glucose 50%. Further in vivo investigations are suggested.