Thyroid Hormone Status Interferes with Estrogen Target Gene Expression in Breast Cancer Samples in Menopausal Women
Figure 3
Gene expression of known thyroid hormone-stimulated genes in primary cultures of breast tumors. Samples were treated as follows: ethanol vehicle, triiodothyronine (T3), T3 plus tamoxifen (TAM), TAM, estradiol (E2), and E2 plus TAM. After 48 h of treatment, THRA, TNFRSF9, and BMP6 were quantified by real-time RT-PCR. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used to normalize gene expression. Relative mRNA expression was calculated using the expression level in the treated ethanol sample as the standard set to the dotted line and represented by letter “a.” Different letters indicate .
