Expression of a Mutant kcnj2 Gene Transcript in Zebrafish
Figure 1
Average relative temporal and spatial gene expression and whole mount in situ hybridisation staining for kcnj2-12. (a) Average spatial gene expression profile. (b) Average temporal expression profile during embryonic development. Results for (a) and (b) were averaged across three experiments. The relative mRNA expression is quantified by qRT-PCR and all mRNA expression is relative to housekeeper genes ef1α and rpl13α. The error bars represent standard errors of the means. The tissues for each spatial gene expression profile were pooled from ten fish and 20–25 embryos were pooled for each time point of the temporal expression profile. (c) Left: side view of 24 hpf embryo showing staining throughout the whole of the body with the most staining in the brain, eyes, and somites (40x magnification; the black arrowhead indicated somite staining). Middle: a magnified side view of the defined staining in the somites (100x magnification). Right top: a magnified frontal view of the eyes and neurotube (100x magnification). Left bottom: dorsal view of staining of the otic vesicles and the somites (100x magnification; indicated by the white arrowheads). (d) Left: (top) dorsal view and (bottom) side view of 48 hpf stained embryo. Staining is strong in the brain, eyes, and slightly in the heart (40x magnification; heart staining indicated by a pink arrowhead and faint staining in the trunk indicated by a black arrowhead). Right: magnified view of the heart (100x magnification).
