Table of Contents
ISRN Molecular Biology
Volume 2014 (2014), Article ID 324839, 14 pages
http://dx.doi.org/10.1155/2014/324839
Research Article

Expression of a Mutant kcnj2 Gene Transcript in Zebrafish

1School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand
2Greenlane Paediatric and Congenital Cardiac Service, Starship Children’s Hospital, Private Bag 92024 Grafton, Auckland 1142, New Zealand
3Department of Obstetrics and Gynaecology, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand
4Diagnostic Genetics, LabPlus, Auckland City Hospital, P.O. Box 110031, Auckland 1142, New Zealand

Received 26 September 2013; Accepted 20 October 2013; Published 12 January 2014

Academic Editors: H. Yu and C.-H. Yuh

Copyright © 2014 Ivone U. S. Leong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. Tawil, L. J. Ptacek, S. G. Pavlakis et al., “Andersen's syndrome: potassium-sensitive periodic paralysis, ventricular ectopy, and dysmorphic features,” Annals of Neurology, vol. 35, no. 3, pp. 326–330, 1994. View at Publisher · View at Google Scholar · View at Scopus
  2. A. Llobet, X. Gasull, J. Palés, E. Martí, and A. Gual, “Identification of kir2.1 channel activity in cultured trabecular meshwork cells,” Investigative Ophthalmology and Visual Science, vol. 42, no. 10, pp. 2371–2379, 2001. View at Google Scholar · View at Scopus
  3. K. F. Raab-Graham, C. M. Radeke, and C. A. Vandenberg, “Molecular cloning and expression of a human heart inward rectifier potassium channel,” NeuroReport, vol. 5, no. 18, pp. 2501–2505, 1994. View at Publisher · View at Google Scholar · View at Scopus
  4. J. B. Redell and B. L. Tempel, “Multiple promoter elements interact to control the transcription of the potassium channel gene, KCNJ2,” Journal of Biological Chemistry, vol. 273, no. 35, pp. 22807–22818, 1998. View at Publisher · View at Google Scholar · View at Scopus
  5. J. J. Zaritsky, D. M. Eckman, G. C. Wellman, M. T. Nelson, and T. L. Schwarz, “Targeted disruption of kir2.1 and kir2.2 genes reveals the essential role of the inwardly rectifying k+ current in k+-mediated vasodilation,” Circulation Research, vol. 87, no. 2, pp. 160–166, 2000. View at Publisher · View at Google Scholar · View at Scopus
  6. J. J. Zaritsky, J. B. Redell, B. L. Tempel, and T. L. Schwarz, “The consequences of disrupting cardiac in wardly rectifying k+ current (IK1) as revealed by the targeted deletion of the murine kir2.1 and kir2.2 genes,” Journal of Physiology, vol. 533, no. 3, pp. 697–710, 2001. View at Publisher · View at Google Scholar · View at Scopus
  7. J. Li, M. McLerie, and A. N. Lopatin, “Transgenic upregulation of IK1 in the mouse heart leads to multiple abnormalities of cardiac excitability,” American Journal of Physiology: Heart and Circulatory Physiology, vol. 287, no. 6, pp. H2790–H2802, 2004. View at Publisher · View at Google Scholar · View at Scopus
  8. C. W. Lu, J. H. Lin, Y. S. Rajawat et al., “Functional and clinical characterization of a mutation in KCNJ2 associated with Andersen-Tawil syndrome,” Journal of Medical Genetics, vol. 43, no. 8, pp. 653–659, 2006. View at Publisher · View at Google Scholar · View at Scopus
  9. M. McLerie and A. Lopatin, “Dominant-negative suppression of IK1 in the mouse heart leads to altered cardiac excitability,” Journal of Molecular and Cellular Cardiology, vol. 35, no. 4, pp. 367–378, 2003. View at Publisher · View at Google Scholar · View at Scopus
  10. A. Dodd, P. M. Curtis, L. C. Williams, and D. R. Love, “Zebrafish: bridging the gap between development and disease,” Human Molecular Genetics, vol. 9, no. 16, pp. 2443–2449, 2000. View at Publisher · View at Google Scholar · View at Scopus
  11. K. Dooley and L. I. Zon, “Zebrafish: a model system for the study of human disease,” Current Opinion in Genetics and Development, vol. 10, no. 3, pp. 252–256, 2000. View at Publisher · View at Google Scholar · View at Scopus
  12. F. Brette, G. Luxan, C. Cros, H. Dixey, C. Wilson, and H. A. Shiels, “Characterization of isolated ventricular myocytes from adult Zebrafish (Danio rerio),” Biochemical and Biophysical Research Communications, vol. 374, no. 1, pp. 143–146, 2008. View at Publisher · View at Google Scholar · View at Scopus
  13. N. Hu, D. Sedmera, H. J. Yost, and E. B. Clark, “Structure and function of the developing Zebrafish heart,” The Anatomical Record, vol. 260, no. 2, pp. 148–157, 2000. View at Google Scholar
  14. D. J. Milan, I. L. Jones, P. T. Ellinor, and C. A. MacRae, “In vivo recording of adult Zebrafish electrocardiogram and assessment of drug-induced QT prolongation,” American Journal of Physiology: Heart and Circulatory Physiology, vol. 291, no. 1, pp. H269–H273, 2006. View at Publisher · View at Google Scholar · View at Scopus
  15. P. Nemtsas, E. Wettwer, T. Christ, G. Weidinger, and U. Ravens, “Adult Zebrafish heart as a model for human heart? an electrophysiological study,” Journal of Molecular and Cellular Cardiology, vol. 48, no. 1, pp. 161–171, 2010. View at Publisher · View at Google Scholar · View at Scopus
  16. D. Sedmera, M. Reckova, A. DeAlmeida et al., “Functional and morphological evidence for a ventricular conduction system in Zebrafish and xenopus hearts,” American Journal of Physiology: Heart and Circulatory Physiology, vol. 284, no. 4, pp. H1152–H1160, 2003. View at Publisher · View at Google Scholar · View at Scopus
  17. R. Arnaout, T. Ferrer, J. Huisken et al., “Zebrafish model for human long QT syndrome,” Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 27, pp. 11316–11321, 2007. View at Publisher · View at Google Scholar · View at Scopus
  18. U. Langheinrich, G. Vacun, and T. Wagner, “Zebrafish embryos express an orthologue of HERG and are sensitive toward a range of QT-prolonging drugs inducing severe arrhythmia,” Toxicology and Applied Pharmacology, vol. 193, no. 3, pp. 370–382, 2003. View at Publisher · View at Google Scholar · View at Scopus
  19. B. Meder, E. P. Scholz, D. Hassel et al., “Reconstitution of defective protein trafficking rescues long-QT syndrome in Zebrafish,” Biochemical and Biophysical Research Communications, vol. 408, no. 2, pp. 218–224, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. S. W. Mittelstadt, C. L. Hemenway, M. P. Craig, and J. R. Hove, “Evaluation of Zebrafish embryos as a model for assessing inhibition of hERG,” Journal of Pharmacological and Toxicological Methods, vol. 57, no. 2, pp. 100–105, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. D. S. Peal, R. W. Mills, S. N. Lynch et al., “Novel chemical suppressors of long QT syndrome identified by an in vivo functional screen,” Circulation, vol. 123, no. 1, pp. 23–30, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. D. P. Wall, H. B. Fraser, and A. E. Hirsh, “Detecting putative orthologs,” Bioinformatics, vol. 19, no. 13, pp. 1710–1711, 2003. View at Publisher · View at Google Scholar · View at Scopus
  23. K. Tamura, J. Dudley, M. Nei, and S. Kumar, “MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0,” Molecular Biology and Evolution, vol. 24, no. 8, pp. 1596–1599, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. R. Tang, A. Dodd, D. Lai, W. C. McNabb, and D. R. Love, “Validation of Zebrafish (Danio rerio) reference genes for quantitative real-time RT-PCR normalization,” Acta Biochimica et Biophysica Sinica, vol. 39, no. 5, pp. 384–390, 2007. View at Publisher · View at Google Scholar · View at Scopus
  25. I. U. S. Leong, J. R. Skinner, A. N. Shelling, and D. R. Love, “Identification and expression analysis of kcnh2 genes in the Zebrafish,” Biochemical and Biophysical Research Communications, vol. 396, no. 4, pp. 817–824, 2010. View at Publisher · View at Google Scholar · View at Scopus
  26. C.-C. Lan, R. Tang, I. U. S. Leong, and D. R. Love, “Quantitative real-time RT-PCR (qRT-PCR) of Zebrafish transcripts: optimization of RNA extraction, quality control considerations, and data analysis,” Cold Spring Harbor Protocols, vol. 4, prot 5314, no. 10, 2009. View at Publisher · View at Google Scholar · View at Scopus
  27. A. Ståhlberg, P. Åman, B. Ridell, P. Mostad, and M. Kubista, “Quantitative real-time PCR method for detection of b-lymphocyte monoclonality by comparison of κ and λ immunoglobulin light chain expression,” Clinical Chemistry, vol. 49, no. 1, pp. 51–59, 2003. View at Publisher · View at Google Scholar · View at Scopus
  28. J. Hellemans, G. Mortier, A. De Paepe, F. Speleman, and J. Vandesompele, “qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data,” Genome Biology, vol. 8, no. 2, article R19, 2007. View at Publisher · View at Google Scholar · View at Scopus
  29. C. Thisse and B. Thisse, “High-resolution in situ hybridization to whole-mount Zebrafish embryos,” Nature Protocols, vol. 3, no. 1, pp. 59–69, 2008. View at Publisher · View at Google Scholar · View at Scopus
  30. M. B. Walker and C. B. Kimmel, “A two-color acid-free cartilage and bone stain for Zebrafish larvae,” Biotechnic and Histochemistry, vol. 82, no. 1, pp. 23–28, 2007. View at Publisher · View at Google Scholar · View at Scopus
  31. M. D. Abramoff, P. J. Magalhaes, and S. J. Ram, “Image processing with IMageJ,” Biophotonics International, vol. 1, pp. 36–42, 2004. View at Google Scholar
  32. H. Hibino, A. Inanobe, K. Furutani, S. Murakami, I. Findlay, and Y. Kurachi, “Inwardly rectifying potassium channels: their structure, function, and physiological roles,” Physiological Reviews, vol. 90, no. 1, pp. 291–366, 2010. View at Publisher · View at Google Scholar · View at Scopus
  33. W. B. Barbazuk, I. Korf, C. Kadavi et al., “The syntenic relationship of the Zebrafish and human genomes,” Genome Research, vol. 10, no. 9, pp. 1351–1358, 2000. View at Publisher · View at Google Scholar · View at Scopus
  34. C. B. Kimmel, W. W. Ballard, S. R. Kimmel, B. Ullmann, and T. F. Schilling, “Stages of embryonic development of the Zebrafish,” Developmental Dynamics, vol. 203, no. 3, pp. 253–310, 1995. View at Publisher · View at Google Scholar · View at Scopus
  35. M. Tristani-Firouzi, J. L. Jensen, M. R. Donaldson et al., “Functional and clinical characterization of KCNJ2 mutations associated with LQT7 (Andersen syndrome),” Journal of Clinical Investigation, vol. 110, no. 3, pp. 381–388, 2002. View at Publisher · View at Google Scholar · View at Scopus
  36. J. Burrone, M. O'Byrne, and V. N. Murthy, “Multiple forms of synaptic plasticity triggered by selective suppression of activity in individual neurons,” Nature, vol. 420, no. 6914, pp. 414–418, 2002. View at Publisher · View at Google Scholar · View at Scopus
  37. J. Y. Hua, M. C. Smear, H. Baier, and S. J. Smith, “Regulation of axon growth in vivo by activity-based competition,” Nature, vol. 434, no. 7036, pp. 1022–1026, 2005. View at Publisher · View at Google Scholar · View at Scopus
  38. T. Yoshida, S. Uchida, and M. Mishina, “Regulation of synaptic vesicle accumulation and axon terminal remodeling during synapse formation by distinct Ca2+ signaling,” Journal of Neurochemistry, vol. 111, no. 1, pp. 160–170, 2009. View at Publisher · View at Google Scholar · View at Scopus
  39. N. M. Plaster, R. Tawil, M. Tristani-Firouzi et al., “Mutations in kir2.1 cause the developmental and episodic electrical phenotypes of Andersen's syndrome,” Cell, vol. 105, no. 4, pp. 511–519, 2001. View at Publisher · View at Google Scholar · View at Scopus
  40. K. A. McKeown, G. B. Downes, and L. D. Hutson, “Modular laboratory exercises to analyze the development of Zebrafish motor behavior,” Zebrafish, vol. 6, no. 2, pp. 179–185, 2009. View at Publisher · View at Google Scholar · View at Scopus
  41. Y. Naganawa and H. Hirata, “Developmental transition of touch response from slow muscle-mediated coilings to fast muscle-mediated burst swimming in Zebrafish,” Developmental Biology, vol. 355, no. 2, pp. 194–204, 2011. View at Publisher · View at Google Scholar · View at Scopus
  42. M. J. Airhart, D. H. Lee, T. D. Wilson, B. E. Miller, M. N. Miller, and R. G. Skalko, “Movement disorders and neurochemical changes in Zebrafish larvae after bath exposure to fluoxetine (PROZAC),” Neurotoxicology and Teratology, vol. 29, no. 6, pp. 652–664, 2007. View at Publisher · View at Google Scholar · View at Scopus
  43. M. Dong, Y. F. Fu, T. T. Du et al., “Heritable and lineage-specific gene knockdown in Zebrafish embryo,” PLoS One, vol. 4, no. 7, Article ID e6125, 2009. View at Publisher · View at Google Scholar · View at Scopus
  44. T. F. Schilling and C. B. Kimmel, “Musculoskeletal patterning in the pharyngeal segments of the Zebrafish embryo,” Development, vol. 124, no. 15, pp. 2945–2960, 1997. View at Google Scholar · View at Scopus
  45. M. L. Suster, H. Kikuta, A. Urasaki, K. Asakawa, and K. Kawakami, “Transgenesis in Zebrafish with the Tol2 transposon system,” Methods in Molecular Biology, vol. 561, pp. 41–63, 2009. View at Publisher · View at Google Scholar · View at Scopus
  46. Y. Doyon, J. M. McCammon, J. C. Miller et al., “Heritable targeted gene disruption in Zebrafish using designed zinc-finger nucleases,” Nature Biotechnology, vol. 26, no. 6, pp. 702–708, 2008. View at Publisher · View at Google Scholar · View at Scopus