Table of Contents
ISRN Molecular Biology
Volume 2014, Article ID 324839, 14 pages
http://dx.doi.org/10.1155/2014/324839
Research Article

Expression of a Mutant kcnj2 Gene Transcript in Zebrafish

1School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand
2Greenlane Paediatric and Congenital Cardiac Service, Starship Children’s Hospital, Private Bag 92024 Grafton, Auckland 1142, New Zealand
3Department of Obstetrics and Gynaecology, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand
4Diagnostic Genetics, LabPlus, Auckland City Hospital, P.O. Box 110031, Auckland 1142, New Zealand

Received 26 September 2013; Accepted 20 October 2013; Published 12 January 2014

Academic Editors: H. Yu and C.-H. Yuh

Copyright © 2014 Ivone U. S. Leong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary materials contain additional figures relating to the bioinformatic analysis and expression of WT and Δ95–98 kcnj2-12 proteins in zebrafish embryos sections. The bioinformatic analyses include the conservation of synteny, alignment of the amino acid sequences of human KCNJ2, mouse Kcnj2 and the five putative zebrafish kcnj2 orthologues, and the unrooted gene tree for the KCNJ2, KCNJ4, KCNJ12 and KCNJ14 gene families. The expression of WT and Δ95–98 kcnj2-12 proteins in zebrafish embryos section include the results of the proportion of dead embryos detected 24 hours after injecting different amounts of WT kcnj2-12 RNA and Δ95–98 kcnj2-12 RNA, the average percentage of death and percentage of deformed embryos expressing Δ95–98 kcnj2-12, WT kcnj2-12 and combined Δ95–98 and WT kcnj2-12 proteins, and images of aberrant embryos expressing Δ95–98 alone, and Δ95–98 and WT protein combined.

The supplemental tables include the Ensembl gene codes of all amino acid sequences used to construct the KCNJ2 unrooted gene tree; the primer sequences used for the verification of the zebrafish orthologue of the human KCNJ2 gene, 5’ RACE of the kcnj2-12 gene transcript, qRT-PCR and construction of the kcnj2-12 recombinant; and the transcript and peptide lengths of all five putative zebrafish kcnj2 gene orthologues.

  1. Supplementary Material