Effect of Analgesics on Monoclonal Antibody Ascites Production in Mice Administered Upon Recognition of Pain

Table 1

Intervention scoresheet for mice used for mAb ascites production.

Parameter	Observation	Score
Appearance	Normal (coats smooth, eyes/nose clear)	0
	Reduced grooming	1
	Dull/rough coat, ocular/nasal discharge	2
	Absence of grooming, piloerection, hunched up	3
Natural behavior	Normal	0
	Minor changes, less peer interaction	1
	Little peer interaction, less mobile and alert, isolated	2
	No peer interaction, vocalization, self-mutilation, restless or still	3
Provoked behavior	Normal	0
	Subdued but normal when stimulated	1
	Subdued even when stimulated	2
	Unresponsive when stimulated, weak, precomatose	3
	Total	0–9
Total score	Observation frequency or intervention
0	Daily observation (1X)
1-2	Observation  2X daily, at least 4 hours apart
≥2	Start treatment according to group
3–7	Observation  3X daily, at least 4 hours apart
8-9	Euthanize