Effect of Analgesics on Monoclonal Antibody Ascites Production in Mice Administered Upon Recognition of Pain

Table 4

mAb 2F9 ascites volumes, ELISA titers, and ADE titers at midpoint for treatment groups.

Group, Cage ID	Tap no.	No. mice	Total vol. (mL)	Volume mL/mouse	Ab conc. (mg IgG/mL)^a	Avg Ab conc. (mg IgG/mL)^b	ADE titer (ng IgG/mL)^c	Avg ADE titer (ng IgG/mL)^d	ADE/IgG^e
A saline
A1	1	6	8.5	1.4	8.6±1.96	6.7±2.46	152.9	150.8	22.4
A2	1	6	7.0	1.2	4.8±0.40		148.8
A1	2	6	11.0	1.8	26.5±4.08	23.6±4.36	185.1	176.4	7.5
A2	2	6	12.5	2.1	20.7±2.41		167.7
B meloxicam
B1	1	6	10.0	1.7	8.0±1.75	8.1±1.23	162.7	169.7	20.9
B2	1	5^f	9.5	1.9	8.2±0.82		176.8
B1	2	6	8.5	1.4	32.1±4.14	33.2±4.79	201.0	185.9	5.6
B2	2	4^g	6.5	1.6	34.2±6.07		170.8
C buprenorphine
C1	1	5^h	7.5	1.5	9.2±5.11	8.5±3.73	273.3	242.9	28.7
C2	1	6	10.5	1.8	7.7±2.66		212.5
C1	2	4^i	4.5	1.1	29.5±5.23	26.7±5.15	208.5	226.2	8.5
C2	2	6	10.0	1.7	23.8±3.81		243.9
D meloxicam + buprenorphine
D1	1	6	12.5	2.1	9.8±0.40	7.8±2.23	179.4	173.3	22.2
D2	1	6	12.0	2.0	5.8±0.41		167.2
D1	2	6	5.0	0.8	37.1±7.76	31.0±8.60	191.6	173.2	5.6
D2	2	6	7.0	1.2	24.8±3.54		154.8

aAverage anti-PA IgG ELISA titer and standard deviation for each collection (tap number) from three ELISAs. ^bAverage ELISA titer and standard deviation for the first or second collection of ascites for each test group. ^cAverage ADE titer for each cage by collection (tap number) from two assays. ^dAverage ADE titer for the first and second collection of ascites for each test group. ^eRatio between ADE titer and respective anti-PA IgG ELISA titer. ^f1 mouse succumbed while anesthetized after first paracentesis on day 5. ^g1 mouse succumbed on day 7 prior to the second ascites collection. ^h1 mouse succumbed on day 4 before initial paracentesis. ⁱ1 mouse succumbed on day 7 prior to the second ascites collection.