Development and Validation of High Performance Liquid Chromatography Method for Simultaneous Estimation of Flavonoid Glycosides in Withania somnifera Aerial Parts

Table 1

Results of system suitability tests^a.

Parameter	1	2	3
𝑘^′	2.830	3.047	3.517
𝐴𝑆	1.217	1.383	1.035
𝑁	9478	12265	8339
𝑅𝑆	2.53	1.43	2.73
RRT	1.0	1.06	1.18
RSD (Rt)	0.11	0.14	0.16
RSD (AUC)	0.81	1.17	0.40

aTests performed as per USP; Rt: Retention time; 𝑘^′: capacity factor; 𝐴_𝑆: symmetry factor; 𝑁: number of theoretical plates; 𝑅_𝑆: resolution; RSD (Rt): RSD of the retention time; RSD (AUC): RSD of the peak area.