Table of Contents
International Scholarly Research Notices
Volume 2014 (2014), Article ID 380278, 5 pages
Research Article

Effect of Nanosilver Gel, Chlorhexidine Gluconate, and Camphorated Phenol on Enterococcus faecalis Biofilm

Department of Conservative Dentistry and Endodontics, Unit I, Second Affiliated Hospital of Jiamusi University, No. 522 Hong Qi Road, Jiamusi, Heilongjiang 154002, China

Received 6 March 2014; Revised 25 July 2014; Accepted 25 July 2014; Published 19 October 2014

Academic Editor: Willie Peijnenburg

Copyright © 2014 Dong Bo and Cecilia Marcellino Kayombo. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aim. To assess the effectiveness of nanosilver gel (NSG) in comparison to chlorhexidine gluconate (CHX) and camphorated phenol (CP) against Enterococcus faecalis (E.f) biofilm. Methods and Materials. Two tests were done, methyl thiazolyl tetrazolium (MTT) assay and confocal laser scanning microscopy (CLSM) analysis, to determine the effectiveness of NSG, CHX, and CP on E.f biofilm. Polystyrene microtiter 96- and 6-well plates were used for MTT and CLSM, respectively. Nanosilver gel was in three concentrations (0.05%, 0.1%, and 0.2%), chlorhexidine gluconate used was 2%, and camphorated phenol and normal saline were as control. Analysis was done using one-way ANOVA; the post hoc test was run for multiple comparisons. The level of statistical significance was set at . Results. One-way ANOVA showed significant differences among groups (0.05% NSG and CP, 0.1% NSG and CP, 0.2% NSG and CP, 0.1% NSG and 2% CHX, 0.2% and NSG and 2% CHX) () and also showed significant difference between groups (), -ratio 87.823. A post hoc Tukey’s test revealed no significant difference between chlorhexidine gluconate and 0.05% nanosilver gel (). Conclusions. 0.1% and 0.2% nanosilver gel is more effective on Enterococcus faecalis biofilm as compared to chlorhexidine gluconate and camphorated phenol.