Table of Contents
International Scholarly Research Notices
Volume 2014, Article ID 412704, 6 pages
http://dx.doi.org/10.1155/2014/412704
Research Article

Determination of Magnesium Valproate and Its Process Related Impurities by Ultraperformance Liquid Chromatography

National Facility for Drug Discovery through NCEs Development & Instrumentation Support to SMPEs, Department of Chemistry, Saurashtra University, Rajkot, Gujarat 360005, India

Received 23 March 2014; Revised 27 April 2014; Accepted 28 April 2014; Published 14 July 2014

Academic Editor: Luigi Janiri

Copyright © 2014 Rakshit Thakkar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A selective ultraperformance liquid chromatographic (UPLC) method for the determination of magnesium valproate and its process related impurities has been developed. The method includes reversed-phase Acquity BEH C18 column with 100 mm × 2.1 mm i.d. and 1.7 µ particle size. The mobile phase consists of acetonitrile and 5 mM ammonium dihydrogen orthophosphate with pH = 3.0 at 45 : 55 isocratic elution. The flow rate was set at 0.3 mL/min and UV detection was performed at 215 nm. A system suitability test (SST) was developed to govern the quality of the separation. The developed method has been validated further with respect to linearity, accuracy, precision, selectivity, LOD, LOQ, and robustness. Different batches of samples were examined using this method; the method proved to be successful when applied to analyze a marketed magnesium valproate formulation.