Optical Fiber-Based Steady State and Fluorescence Lifetime Spectroscopy for Rapid Identification and Classification of Bacterial Pathogens Directly from Colonies on Agar Plates

Table 2

The fast (), intermediate (), and slow () lifetime components and their relative amplitudes from S. aureus, P. aeruginosa, S. typhi, and K. Pneumoniae at 280 nm excitation.

Sample	Fast component		Intermediate component		Slow component		CHISQ
	(ns)	(%)	(ns)	(%)	(ns)	(%)
S. aureus	0.19 ± 0.02	0.24 ± 0.02	1.68 ± 0.07	0.26 ± 0.02	4.16 ± 0.09	0.50 ± 0.01	1.08 ± 0.05
P. aeruginosa	0.18 ± 0.03	0.27 ± 0.01	1.60 ± 0.10	0.25 ± 0.02	4.57 ± 0.07	0.48 ± 0.02	1.05 ± 0.06
S. typhi	0.21 ± 0.03	0.28 ± 0.06	1.86 ± 0.08	0.33 ± 0.06	5.02 ± 0.10	0.39 ± 0.02	1.06 ± 0.05
K.  pneumoniae	0.29 ± 0.04	0.24 ± 0.01	2.17 ± 0.12	0.34 ± 0.02	5.65 ± 0.05	0.42 ± 0.02	1.05 ± 0.06

(ns): nanosecond.