Table of Contents
ISRN Virology
Volume 2014, Article ID 450361, 8 pages
http://dx.doi.org/10.1155/2014/450361
Research Article

In Silico Studies on Development of Novel Virostatic Agents against Bluetongue Virus

Department of Chemistry, Nizam College, Basheerbagh, Hyderabad 500 001, India

Received 9 December 2013; Accepted 15 January 2014; Published 4 March 2014

Academic Editors: J. Ortego and J. Stephenson

Copyright © 2014 Pandrangi Anupama. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The core of BTV is organized into three concentric structures of which VP7 protein forms the major core protein. The subcore consists of VP3 protein and the innermost part of the core is made of three minor proteins: VP1, VP4, and VP6. Earlier it was reported that core-like particles (CLPs) composed of viral VP7 and VP3 proteins were produced in order to study role of VP7 protein in intermolecular interactions in the BTV assembly process. Site specific mutational studies revealed that substitution of the single lysine residue of VP7 (Lys-255) by leucine abrogated CLP formation, indicating a critical role for this lysine. In the present study, homology modeling, mutagenesis, and docking studies were carried out in order to design potent leads in modulation of VP7 protein in abrogating CLP formation.