In Vitro Intestinal Permeability Studies and Pharmacokinetic Evaluation of Famotidine Microemulsion for Oral Delivery

Table 1

Chromatographic condition for pharmacokinetic study.

	Chromatographic conditions
LC-MS/MS	API 3200, MDS SCIEX
Ionization source	ESI
Column	Agilent Zorbax SB-CN (50 mm × 2.1 mm I.D., 5 micron)
Mobile phase	Methanol: 20 mM ammonium acetate (55 : 45, v/v)
Flow rate	Isocratic flow rate of 0.6 mL/min
Detection	MS/MS
Extraction solvent	3 mL of ethyl acetate
Injection volume	10 L
Retention time of famotidine	1.37 min
Linearity range	5 to 5000 ng/mL
Rabbit plasma volume used	50 L