Table of Contents
International Scholarly Research Notices
Volume 2014, Article ID 464136, 8 pages
http://dx.doi.org/10.1155/2014/464136
Research Article

Andrographolide Exerts Chondroprotective Activity in Equine Cartilage Explant and Suppresses Interleukin-1β-Induced MMP-2 Expression in Equine Chondrocyte Culture

1Department of Companion Animal and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand
2Thailand Excellence Centre for Tissue Engineering and Stem Cells, Department of Biochemistry and the Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand

Received 25 July 2014; Revised 3 October 2014; Accepted 3 October 2014; Published 30 October 2014

Academic Editor: Shuhui Sun

Copyright © 2014 Siriwan Tangyuenyong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. Krasnokutsky, J. Samuels, and S. B. Abramson, “Osteoarthritis in 2007,” Bulletin of the NYU Hospital for Joint Diseases, vol. 65, no. 3, pp. 222–228, 2007. View at Google Scholar · View at Scopus
  2. P. Lees, “Pharmacology of drugs used to treat osteoarthritis in veterinary practice,” Inflammopharmacology, vol. 11, no. 4–6, pp. 385–399, 2003. View at Publisher · View at Google Scholar · View at Scopus
  3. L. Wallin, E. Strandberg, J. Philipsson, and G. Dalin, “Estimates of longevity and causes of culling and death in Swedish warmblood and coldblood horses,” Livestock Production Science, vol. 63, no. 3, pp. 275–289, 2000. View at Publisher · View at Google Scholar · View at Scopus
  4. L. R. Goodrich and A. J. Nixon, “Medical treatment of osteoarthritis in the horse—a review,” The Veterinary Journal, vol. 171, no. 1, pp. 51–69, 2006. View at Publisher · View at Google Scholar · View at Scopus
  5. M. B. Goldring and K. B. Marcu, “Cartilage homeostasis in health and rheumatic diseases,” Arthritis Research & Therapy, vol. 11, no. 3, p. 224, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. M. Kobayashi, G. R. Squires, A. Mousa et al., “Role of interleukin-1 and tumor necrosis factor α in matrix degradation of human osteoarthritic cartilage,” Arthritis and Rheumatism, vol. 52, no. 1, pp. 128–135, 2005. View at Publisher · View at Google Scholar · View at Scopus
  7. M. Yaron, I. Shirazi, and I. Yaron, “eAnti-interleukin-1 effects of diacerein and rhein in human osteoarthritic synovial tissue and cartilage cultures,” Osteoarthritis and Cartilage, vol. 7, no. 3, pp. 272–280, 1999. View at Publisher · View at Google Scholar · View at Scopus
  8. B.-F. Lin, W.-W. Chao, Y.-H. Kuo, and S.-L. Hsieh, “Inhibitory effects of ethyl acetate extract of Andrographis paniculata on NF-κB trans-activation activity and LPS-induced acute inflammation in mice,” Evidence-Based Complementary and Alternative Medicine, vol. 2011, Article ID 254531, 9 pages, 2011. View at Publisher · View at Google Scholar · View at Scopus
  9. R. A. Burgos, J. L. Hancke, J. C. Bertoglio et al., “Efficacy of an Andrographis paniculata composition for the relief of rheumatoid arthritis symptoms: a prospective randomized placebo-controlled trial,” Clinical Rheumatology, vol. 28, no. 8, pp. 931–946, 2009. View at Publisher · View at Google Scholar · View at Scopus
  10. C.-Y. Chao, C.-K. Lii, Y.-T. Hsu et al., “Induction of heme oxygenase-1 and inhibition of TPA-induced matrix metalloproteinase-9 expression by andrographolide in MCF-7 human breast cancer cells,” Carcinogenesis, vol. 34, no. 8, pp. 1843–1851, 2013. View at Publisher · View at Google Scholar · View at Scopus
  11. W. Parichatikanond, C. Suthisisang, P. Dhepakson, and A. Herunsalee, “Study of anti-inflammatory activities of the pure compounds from Andrographis paniculata (burm.f.) Nees and their effects on gene expression,” International Immunopharmacology, vol. 10, no. 11, pp. 1361–1373, 2010. View at Publisher · View at Google Scholar · View at Scopus
  12. Q. H. Ding, X. W. Ji, Y. Cheng, Y. G. Yu, Y. Y. Qi, and X. H. Wang, “Inhibition of matrix metalloproteinases and inducible nitric oxide synthase by andrographolide in human osteoarthritic chondrocytes,” Modern Rheumatology, vol. 23, no. 6, pp. 1124–1132, 2013. View at Publisher · View at Google Scholar · View at Scopus
  13. T. Phitak, K. Choocheep, P. Pothacharoen, W. Pompimon, B. Premanode, and P. Kongtawelert, “The effects of p-hydroxycinnamaldehyde from Alpinia galanga extracts on human chondrocytes,” Phytochemistry, vol. 70, no. 2, pp. 237–243, 2009. View at Publisher · View at Google Scholar · View at Scopus
  14. R. Chaiwongsa, S. Ongchai, S. Tangyuenyong, P. Kongtawelert, A. Panthong, and V. Reutrakul, “Chondroprotective potential of bioactive compounds of Zingiber cassumunar Roxb. against cytokine-induced cartilage degradation in explant culture,” Journal of Medicinal Plants Research, vol. 6, no. 39, pp. 5204–5213, 2012. View at Publisher · View at Google Scholar
  15. D. E. Kleiner and W. G. Stetler-Stevenson, “Quantitative zymography: detection of picogram quantities of gelatinases,” Analytical Biochemistry, vol. 218, no. 2, pp. 325–329, 1994. View at Publisher · View at Google Scholar · View at Scopus
  16. J. Bondeson, S. D. Wainwright, S. Lauder, N. Amos, and C. E. Hughes, “The role of synovial macrophages and macrophage-produced cytokines in driving aggrecanases, matrix metalloproteinases, and other destructive and inflammatory responses in osteoarthritis,” Arthritis Research and Therapy, vol. 8, article R187, 2006. View at Publisher · View at Google Scholar · View at Scopus
  17. Z. Jiang, C. E. Uboh, J. Chen, and L. R. Soma, “Isolation of RNA from equine peripheral blood cells: comparison of methods,” SpringerPlus, vol. 2, article 478, 2013. View at Publisher · View at Google Scholar · View at Scopus
  18. J. A. Glasel, “Validity of nucleic acid purities monitored by 260nm/280nm absorbance ratios,” BioTechniques, vol. 18, no. 1, pp. 62–63, 1995. View at Google Scholar · View at Scopus
  19. S. Fleige and M. W. Pfaffl, “RNA integrity and the effect on the real-time qRT-PCR performance,” Molecular Aspects of Medicine, vol. 27, no. 2-3, pp. 126–139, 2006. View at Publisher · View at Google Scholar · View at Scopus
  20. T. Mosmann, “Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays,” Journal of Immunological Methods, vol. 65, no. 1-2, pp. 55–63, 1983. View at Publisher · View at Google Scholar · View at Scopus
  21. T. Decker and M.-L. Lohmann-Matthes, “A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity,” Journal of Immunological Methods, vol. 115, no. 1, pp. 61–69, 1988. View at Publisher · View at Google Scholar · View at Scopus
  22. T. Aigner, S. Soeder, and J. Haag, “IL-1β and BMPS—interactive players of cartilage matrix degradation and regeneration,” European Cells and Materials, vol. 12, pp. 49–56, 2006. View at Google Scholar · View at Scopus
  23. C. Melchiorri, R. Meliconi, L. Frizziero et al., “Enhanced and coordinated in vivo expression of inflammatory cytokines and nitric oxide synthase by chondrocytes from patients with osteoarthritis,” Arthritis and Rheumatism, vol. 41, no. 12, pp. 2165–2174, 1998. View at Publisher · View at Google Scholar
  24. P. Wojdasiewicz, Ł. A. Poniatowski, and D. Szukiewicz, “The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of osteoarthritis,” Mediators of Inflammation, vol. 2014, Article ID 561459, 19 pages, 2014. View at Publisher · View at Google Scholar · View at Scopus
  25. J. L. Kamm, A. J. Nixon, and T. H. Witte, “Cytokine and catabolic enzyme expression in synovium, synovial fluid and articular cartilage of naturally osteoarthriticequine carpi,” Equine Veterinary Journal, vol. 42, no. 8, pp. 693–699, 2010. View at Publisher · View at Google Scholar · View at Scopus
  26. E. Busschers, J. P. Holt, and D. W. Richardson, “Effects of glucocorticoids and interleukin-1β on expression and activity of aggrecanases in equine chondrocytes,” American Journal of Veterinary Research, vol. 71, no. 2, pp. 176–185, 2010. View at Publisher · View at Google Scholar · View at Scopus
  27. G. Stabellini, M. de Mattei, C. Calastrini et al., “Effects of interleukin-1β on chondroblast viability and extracellular matrix changes in bovine articular cartilage explants,” Biomedicine & Pharmacotherapy, vol. 57, no. 7, pp. 314–319, 2003. View at Publisher · View at Google Scholar · View at Scopus
  28. D. Heinegård, “Proteoglycans and more—from molecules to biology,” International Journal of Experimental Pathology, vol. 90, no. 6, pp. 575–586, 2009. View at Publisher · View at Google Scholar · View at Scopus
  29. D. Heinegård and T. Saxne, “The role of the cartilage matrix in osteoarthritis,” Nature Reviews Rheumatology, vol. 7, no. 1, pp. 50–56, 2011. View at Publisher · View at Google Scholar · View at Scopus
  30. T. Phitak, P. Pothacharoen, and P. Kongtawelert, “Comparison of glucose derivatives effects on cartilage degradation,” BMC Musculoskeletal Disorders, vol. 11, article 162, 2010. View at Publisher · View at Google Scholar · View at Scopus
  31. G. S. Butler, M. J. Butler, S. J. Atkinson et al., “The TIMP2 membrane type 1 metalloproteinase “receptor” regulates the concentration and efficient activation of progelatinase A. A kinetic study,” The Journal of Biological Chemistry, vol. 273, no. 2, pp. 871–880, 1998. View at Publisher · View at Google Scholar · View at Scopus
  32. S. Fietz, R. Einspanier, S. Hoppner, B. Hertsch, and A. Bondzio, “Determination of MMP-2 and -9 activities in synovial fluid of horses with osteoarthritic and arthritic joint diseases using gelatin zymography and immunocapture activity assays,” Equine Veterinary Journal, vol. 40, no. 3, pp. 266–271, 2008. View at Publisher · View at Google Scholar · View at Scopus
  33. H. Song, S. H. Ki, S. G. Kim, and A. Moon, “Activating transcription factor 2 mediates matrix metalloproteinase-2 transcriptional activation induced by p38 in breast epithelial cells,” Cancer Research, vol. 66, no. 21, pp. 10487–10496, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. B. Kumar, S. Koul, J. Petersen et al., “p38 Mitogen-activated protein kinase-driven MAPKAPK2 regulates invasion of bladder cancer by modulation of MMP-2 and MMP-9 activity,” Cancer Research, vol. 70, no. 2, pp. 832–841, 2010. View at Publisher · View at Google Scholar · View at Scopus
  35. K. K. Brown, S. A. Heitmeyer, E. B. Hookfin et al., “P38 MAP kinase inhibitors as potential therapeutics for the treatment of joint degeneration and pain associated with osteoarthritis,” Journal of Inflammation, vol. 5, article 22, 2008. View at Publisher · View at Google Scholar · View at Scopus
  36. W. Wang, J. Wang, S.-F. Dong et al., “Immunomodulatory activity of andrographolide on macrophage activation and specific antibody response,” Acta Pharmacologica Sinica, vol. 31, no. 2, pp. 191–201, 2010. View at Publisher · View at Google Scholar · View at Scopus
  37. T. Zhu, D.-X. Wang, W. Zhang et al., “Andrographolide Protects against LPS-Induced Acute Lung Injury by Inactivation of NF-κB,” PLoS ONE, vol. 8, no. 2, Article ID e56407, 2013. View at Publisher · View at Google Scholar · View at Scopus
  38. T. Shen, W. S. Yang, Y.-S. Yi et al., “AP-1/IRF-3 targeted anti-inflammatory activity of andrographolide isolated from Andrographis paniculata,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 210736, 16 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  39. L. C. Tetlow, D. J. Adlam, and D. E. Woolley, “Matrix metalloproteinase and proinflammatory cytokine production by chondrocytes of human osteoarthritic cartilage: associations with degenerative changes,” Arthritis and Rheumatism, vol. 44, no. 3, pp. 585–594, 2001. View at Publisher · View at Google Scholar
  40. M. M. Hardy, K. Seibert, P. T. Manning et al., “Cyclooxygenase 2-dependent prostaglandin E2 modulates cartilage proteoglycan degradation in human osteoarthritis explants,” Arthritis and Rheumatism, vol. 46, no. 7, pp. 1789–1803, 2002. View at Publisher · View at Google Scholar · View at Scopus
  41. G. S. Firestein, J. M. Alvaro-Garcia, and R. Maki, “Quantitative analysis of cytokine gene expression in rheumatoid arthritis,” Journal of Immunology, vol. 144, no. 9, pp. 3347–3353, 1990. View at Google Scholar · View at Scopus