Table of Contents
ISRN Nanotechnology
Volume 2014, Article ID 480284, 8 pages
Research Article

Silver Nanoparticle Synthesis Using Monosaccharides and Their Growth Inhibitory Activity against Gram-Negative and Positive Bacteria

Laboratory of Biochemistry, Northwest Missouri State University, Garrett-Strong Science Building 3100, Maryville, MO 64468, USA

Received 12 November 2013; Accepted 2 January 2014; Published 12 February 2014

Academic Editors: L. A. Dykman, A. N. Obraztsov, and T. Pal

Copyright © 2014 Colin Pettegrew et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Using various monosaccharides as reductant, we synthesized Ag nanoparticles (NPs) in seconds employing the household microwave method described earlier. The Ag NPs containing colloidal solution showed distinctive colors with varying . The sizes of the NPs formed varied significantly from 10 to 35 nm in good agreement with the localized plasmon resonance ranged from ~300 to ~600 nm. The antimicrobial properties of these NPs were compared in Gram-negative and positive bacteria in liquid culture. Gram-positive bacteria were highly susceptible compared to Gram-negative microbes—the additional lipopolysaccharide layer covering the peptidoglycan cell wall in the latter somewhat lessens the effect. The results indicated that larger NPs produced by glucose inhibited bacterial growth better than the smallest NPs produced by ribose. This may be attributed to the higher aggregation rate for larger NPs on cell wall. SEM analysis showed accumulation of NPs on cell surface and defect in budding, further supporting the cell wall interaction with Ag NPs. These observations suggested that the growth inhibition of Ag NPs is mediated by interfering with the bacterial cell wall peptidoglycan.