Research Article

Development and Validation of an Affinity Chromatography-Protein G Method for IgG Quantification

Figure 2

Effects of different factors on the IgG concentration, where A: molarity of mobile phase A, B: molarity of mobile phase B, C: pH of mobile phase A, D: batch of column, E: molarity of mobile phase C, F: pH of mobile phase B, and G: oven temperature.