Development and Validation of an Affinity Chromatography-Protein G Method for IgG Quantification

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Mobile phases B and C molarity (M)</td><td align="center">Tailing factor (%)</td><td align="center">Specification USP tailing</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">0.05</td><td align="center">2.846</td><td align="center" rowspan="4">≤2%</td></tr><tr><td align="left">0.1</td><td align="center">2.581</td></tr><tr><td align="left">0.25</td><td align="center">1.941</td></tr><tr><td align="left">0.5</td><td align="center">1.794</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Comparison between the tailing factor of IgG elution peak for different molarity values in the mobile phases B and C.

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Table 1

Table 1: Development and Validation of an Affinity Chromatography-Protein G Method for IgG Quantification 