Influence of Albizia lebbeck Saponin and Its Fractions on In Vitro Gas Production Kinetics, Rumen Methanogenesis, and Rumen Fermentation Characteristics

Table 4

Effects of A.  lebbeck saponins fractions on volatile fatty acid and ammonia nitrogen.

Parameters

Diets

D1 (60R : 40C)

D2(50R : 50C)

D3(40R : 60C)

SEM

Control

GSE

Sap A

Sap B

Control

GSE

Sap A

Sap B

Control

GSE

Sap A

Sap B

Diet

Treatment

D * T

Acetate; (mM/100 mL)

9.24±0.12

8.13±0.10

8.40±0.16

7.23±0.30

9.19±0.21

9.28±0.10

9.41±0.09

10.62±0.03

8.02±0.13

8.48±0.27

8.40±0.54

8.47±0.32

0.165

0.191

0.330

Propionate (mM/100 mL)

2.53±0.10

2.16±0.08

2.25±0.11

1.93±0.16

2.65±0.11

2.70±0.14

3.00±0.06

3.61±0.04

2.29±0.06

2.19±0.14

2.48±0.28

1.91±0.08

0.064

0.077

0.133

Butyrate; (mM/100 mL)

1.09±0.06

0.89±0.06

0.99±0.02

0.89±0.01

1.02±0.04

0.94±0.07

1.01±0.02

1.18±0.01

1.05±0.04

1.18±0.016

0.92±0.214

0.97±0.05

0.046

0.053

0.092

C2 : C3

3.65±0.12

3.76±0.10

3.74±0.16

3.77±0.30

3.48±0.21

3.44±0.10

3.14±0.09

2.94±0.03

3.50±0.13

3.90±0.27

3.51±0.54

4.47±0.32

0.119

0.137

0.238

NH3-N; (mg/100 mL)

19.23±0.09

18.48±0.16

16.43±0.09

15.49±0.09

17.36±0.16

15.87±0.49

12.13±0.09

10.64±0.16

19.79±0.09

19.13±0.09

16.43±0.09

16.05±0.09

0.092

0.106

0.183

D₁: high fiber diet, D₂: medium fiber diet, D₃: low fiber diet, GSE: gross saponins extract, C2 : C3: acetate to propionate ratio, NH₃-N: ammonia nitrogen, SEM: standard error of means.