Table of Contents
International Scholarly Research Notices
Volume 2014, Article ID 525719, 7 pages
Research Article

Giardia duodenalis: Number and Fluorescence Reduction Caused by the Advanced Oxidation Process (H2O2/UV)

1Department of Sanitation and the Environment, School of Civil Engineering, Architecture and Urbanism, University of Campinas (UNICAMP), CP 6021, 13083-852 Campinas, SP, Brazil
2Department of Animal Biology, Biology Institute, University of Campinas (UNICAMP), CP 6109, 13083-970 Campinas, SP, Brazil
3Padre Anchieta University Center, UNIANCHIETA, Bom Jesus de Pirapora Street 100/140, 13207-270 Jundiaí, SP, Brazil

Received 1 September 2014; Accepted 4 November 2014; Published 7 December 2014

Academic Editor: Erick R. Bandala

Copyright © 2014 José Roberto Guimarães et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study evaluated the effect of peroxidation assisted by ultraviolet radiation (H2O2/UV), which is an advanced oxidation process (AOP), on Giardia duodenalis cysts. The cysts were inoculated in synthetic and surface water using a concentration of 12 g H2O2 L−1 and a UV dose ( nm) of 5,480 mJcm−2. The aqueous solutions were concentrated using membrane filtration, and the organisms were observed using a direct immunofluorescence assay (IFA). The AOP was effective in reducing the number of G. duodenalis cysts in synthetic and surface water and was most effective in reducing the fluorescence of the cyst walls that were present in the surface water. The AOP showed a higher deleterious potential for G. duodenalis cysts than either peroxidation (H2O2) or photolysis (UV) processes alone.