Table of Contents
ISRN Chemical Engineering
Volume 2014, Article ID 536310, 9 pages
Research Article

Effect of Various Pretreatment for Extracting Intracellular Lipid from Nannochloropsis oculata under Nitrogen Replete and Depleted Conditions

Bioelectrochemical Laboratory, Department of Chemical Engineering, Faculty of Engineering and Technology, Annamalai University, Annamalai Nagar, Tamil Nadu 608002, India

Received 25 November 2013; Accepted 7 January 2014; Published 4 March 2014

Academic Editors: M. Hamdi and M. Kostoglou

Copyright © 2014 Duraiarasan Surendhiran and Mani Vijay. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Microalga is one of the most compelling microbial biomasses for biodiesel production. Various pretreatment processes, namely, enzyme treatment, lysis by acid, ultrasonicator, microwaves, autoclave, and 40% NaCl, for nitrogen replete and depleted algal cultures of Nannochloropsis oculata had been carried out to check the most feasible and effective technique to disrupt cells for procuring lipids, for which concentrations were determined. Fatty acid composition, essential functional groups, and cell disruption were analyzed by GC-MS, FT-IR Spectroscopy, and Nile Red fluorescent microscopy, respectively. The present investigation showed that lipid yield was higher in nitrogen depleted cells than that in normally nourished cells. GC-MS revealed the presence of major fatty acids—palmitic, oleic, stearic, arachidic, lauric, and linoleic acids. Highest efficiency was found when cells were pretreated using acid for 3 h. The lipid content was calculated as 33.18% and 54.26% for nitrogen rich cells and nitrogen starved cells, respectively. This work thus aided in identifying the most eligible pretreatment process to avail lipids from cells, to convert them to eco-friendly and nonpolluting biodiesel.