Table of Contents
ISRN Chemical Engineering
Volume 2014, Article ID 536310, 9 pages
http://dx.doi.org/10.1155/2014/536310
Research Article

Effect of Various Pretreatment for Extracting Intracellular Lipid from Nannochloropsis oculata under Nitrogen Replete and Depleted Conditions

Bioelectrochemical Laboratory, Department of Chemical Engineering, Faculty of Engineering and Technology, Annamalai University, Annamalai Nagar, Tamil Nadu 608002, India

Received 25 November 2013; Accepted 7 January 2014; Published 4 March 2014

Academic Editors: M. Hamdi and M. Kostoglou

Copyright © 2014 Duraiarasan Surendhiran and Mani Vijay. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. P. Schlagermann, G. Gottlicher, R. Dillschneider, R. Rosello-Sastre, and C. Posten, “Composition of algal oil and its potential as biofuel,” Journal of Combustion, vol. 2012, Article ID 285185, 14 pages, 2012. View at Publisher · View at Google Scholar
  2. H.-C. Chen, H.-Y. Ju, T.-T. Wu et al., “Continuous production of lipase-catalyzed biodiesel in a packed-bed reactor: optimization and enzyme reuse study,” Journal of Biomedicine and Biotechnology, vol. 2011, Article ID 950725, 6 pages, 2011. View at Publisher · View at Google Scholar · View at Scopus
  3. A. Widjaja, C.-C. Chien, and Y.-H. Ju, “Study of increasing lipid production from fresh water microalgae Chlorella vulgaris,” Journal of the Taiwan Institute of Chemical Engineers, vol. 40, no. 1, pp. 13–20, 2009. View at Publisher · View at Google Scholar · View at Scopus
  4. M. A. Rodrigues and E. P. S. Bon, “Evaluation of chlorella (Chlorophyta) as source of fermentable sugars via cell wall enzymatic hydrolysis,” Enzyme Research, vol. 2011, Article ID 405603, 5 pages, 2011. View at Publisher · View at Google Scholar
  5. E. Ryckebosch, K. Muylaert, and I. Foubert, “Optimization of an analytical procedure for extraction of lipids from microalgae,” Journal of the American Oil Chemists' Society, vol. 89, no. 2, pp. 189–198, 2012. View at Publisher · View at Google Scholar · View at Scopus
  6. H. Zheng, J. Yin, Z. Gao, H. Huang, X. Ji, and C. Dou, “Disruption of Chlorella vulgaris cells for the release of biodiesel-producing lipids: a comparison of grinding, ultrasonication, bead milling, enzymatic lysis, and microwaves,” Applied Biochemistry and Biotechnology, vol. 164, no. 7, pp. 1215–1224, 2011. View at Publisher · View at Google Scholar · View at Scopus
  7. P. Mercer and R. E. Armenta, “Developments in oil extraction from microalgae,” European Journal of Lipid Science and Technology, vol. 113, no. 5, pp. 539–547, 2011. View at Publisher · View at Google Scholar
  8. T. Suganya and S. Renganathan, “Optimization and kinetic studies on algal oil extraction from marine macroalgae Ulva lactuca,” Bioresource Technology, vol. 107, pp. 319–326, 2012. View at Publisher · View at Google Scholar · View at Scopus
  9. K. Sander and G. S. Murthy, “Enzymatic degradation of microalgal cell walls,” in Proceedings of the American Society of Agricultural and Biological Engineers Annual International Meeting (ASABE '09), Paper Number: 1035636, pp. 2489–2500, June 2009. View at Scopus
  10. J.-Y. Lee, C. Yoo, S.-Y. Jun, C.-Y. Ahn, and H.-M. Oh, “Comparison of several methods for effective lipid extraction from microalgae,” Bioresource Technology, vol. 101, no. 1, pp. S75–S77, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. G. Jin, F. Yang, C. Hu, H. Shen, and Z. K. Zhao, “Enzyme-assisted extraction of lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides,” Bioresource Technology, vol. 111, pp. 378–382, 2012. View at Publisher · View at Google Scholar · View at Scopus
  12. E. G. Bligh and W. J. Dyer, “A rapid method of total lipid extraction and purification,” Canadian Journal of Biochemistry and Physiology, vol. 37, no. 8, pp. 911–917, 1959. View at Google Scholar · View at Scopus
  13. N. G.-E. Mohammady, C. W. Rieken, S. R. Lindell et al., “Age of nitrogen deficient microalgal cells is a key factor for maximizing lipid content,” Research Journal of Phytochemistry, vol. 6, no. 2, pp. 42–53, 2012. View at Publisher · View at Google Scholar · View at Scopus
  14. C.-H. Su, C.-C. Fu, Y.-C. Chang et al., “Simultaneous estimation of chlorophyll a and lipid contents in microalgae by three-color analysis,” Biotechnology and Bioengineering, vol. 99, no. 4, pp. 1034–1039, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, “Protein measurement with the Folin phenol reagent,” The Journal of Biological Chemistry, vol. 193, no. 1, pp. 265–275, 1951. View at Google Scholar · View at Scopus
  16. C. Yeesang and B. Cheirsilp, “Effect of nitrogen, salt, and iron content in the growth medium and light intensity on lipid production by microalgae isolated from freshwater sources in Thailand,” Bioresource Technology, vol. 102, no. 3, pp. 3034–3040, 2011. View at Publisher · View at Google Scholar · View at Scopus
  17. M. Alsull and W. M. W. Omar, “Responses of Tetraselmis sp. and Nannochloropsis sp. isolated from Penang National Park coastal waters, Malaysia, to the combined influences of salinity, light and nitrogen limitation,” in Proceedings of the International Conference on Chemical, Ecology and Environmental Sciences (ICEES '12), pp. 142–145, Bangkok, Thailand, March 2012.
  18. C. M. Beal, M. E. Webber, R. S. Ruoff, and R. E. Hebner, “Lipid analysis of Neochloris oleoabundans by liquid state NMR,” Biotechnology and Bioengineering, vol. 106, no. 4, pp. 573–583, 2010. View at Publisher · View at Google Scholar · View at Scopus
  19. D. Feng, Z. Chen, S. Xue, and W. Zhang, “Increased lipid production of the marine oleaginous microalgae Isochrysis zhangjiangensis (Chrysophyta) by nitrogen supplement,” Bioresource Technology, vol. 102, no. 12, pp. 6710–6716, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. G. Ahlgren and P. Hyenstrand, “Nitrogen limitation effects of different nitrogen sources on nutritional quality of two freshwater organisms, Scenedesmus quadricauda (Chlorophyceae) and Synechococcus sp. (Cyanophyceae),” Journal of Phycology, vol. 39, no. 5, pp. 906–917, 2003. View at Google Scholar · View at Scopus
  21. M. Hoffmann, K. Marxen, R. Schulz, and K. H. Vanselow, “TFA and EPA productivities of Nannochloropsis salina influenced by temperature and nitrate stimuli in turbidostatic controlled experiments,” Marine Drugs, vol. 8, no. 9, pp. 2526–2545, 2010. View at Publisher · View at Google Scholar · View at Scopus
  22. S. Elumalai, V. Prakasam, and R. Selvarajan, “Optimization of abiotic conditions suitable for the production of biodiesel from Chlorella vulgaris,” Indian Journal of Science and Technology, vol. 4, no. 2, pp. 91–97, 2011. View at Google Scholar
  23. U. Pick and T. Rachutin-Zalogin, “Kinetic anomalies in the interactions of Nile red with microalgae,” Journal of Microbiological Methods, vol. 88, no. 2, pp. 189–196, 2012. View at Publisher · View at Google Scholar · View at Scopus
  24. M. M. Phukan, R. S. Chutia, B. K. Konwar, and R. Kataki, “Microalgae Chlorella as a potential bio-energy feedstock,” Applied Energy, vol. 88, no. 10, pp. 3307–3312, 2011. View at Google Scholar
  25. N. Rukminasari, “Effect of nutrient depletion and temperature tressed on growth and lipid accumulation in marine-green algae Nannochloropsis sp,” American Journal of Research Communication. In press.
  26. C.-C. Fu, T.-C. Hung, J.-Y. Chen, C.-H. Su, and W.-T. Wu, “Hydrolysis of microalgae cell walls for production of reducing sugar and lipid extraction,” Bioresource Technology, vol. 101, no. 22, pp. 8750–8754, 2010. View at Publisher · View at Google Scholar · View at Scopus
  27. W. L. Zemke-White, K. D. Clements, and P. J. Harris, “Acid lysis of macroalgae by marine herbivorous fishes: effects of acid pH on cell wall porosity,” Journal of Experimental Marine Biology and Ecology, vol. 245, no. 1, pp. 57–68, 2000. View at Publisher · View at Google Scholar · View at Scopus
  28. R. Harun and M. K. Danquah, “Influence of acid pre-treatment on microalgal biomass for bioethanol production,” Process Biochemistry, vol. 46, no. 1, pp. 304–309, 2011. View at Publisher · View at Google Scholar · View at Scopus
  29. C. Dejoye, M. A. Vian, G. Lumia, C. Bouscarle, F. Charton, and F. Chemat, “Combined extraction processes of lipid from Chlorella vulgaris microalgae: microwave prior to supercritical carbon dioxide extraction,” International Journal of Molecular Sciences, vol. 12, no. 12, pp. 9332–9341, 2011. View at Publisher · View at Google Scholar · View at Scopus
  30. S. Li, H. Zhang, D. Han, and K. H. Row, “Optimization of enzymatic extraction of polysaccharides from some marine algae by response surface methodology,” Korean Journal of Chemical Engineering, vol. 29, no. 5, pp. 650–656, 2012. View at Publisher · View at Google Scholar · View at Scopus
  31. G. Huang, F. Chen, D. Wei, X. Zhang, and G. Chen, “Biodiesel production by microalgal biotechnology,” Applied Energy, vol. 87, no. 1, pp. 38–46, 2010. View at Publisher · View at Google Scholar · View at Scopus
  32. N. O. Zhila, G. S. Kalacheva, and T. G. Volova, “Effect of nitrogen limitation on the growth and lipid composition of the green alga Botryococcus braunii Kütz IPPAS H-252,” Russian Journal of Plant Physiology, vol. 52, no. 3, pp. 311–319, 2005. View at Publisher · View at Google Scholar · View at Scopus
  33. Q. Lin, N. Gu, G. Li, J. Lin, L. Huang, and L. Tan, “Effects of inorganic carbon concentration on carbon formation, nitrate utilization, biomass and oil accumulation of Nannochloropsis oculata CS 179,” Bioresource Technology, vol. 111, pp. 353–359, 2012. View at Publisher · View at Google Scholar · View at Scopus