Effect of Various Pretreatment for Extracting Intracellular Lipid from Nannochloropsis oculata under Nitrogen Replete and Depleted Conditions

Table 2

Representing the functional groups of nitrogen replete and depleted cells of N. oculata.

Wave numbers (cm^−1)	Functional groups
3000–2700	C–H stretching vibrations of –CH3, >CH2, CH, CHO
3600–3300	Oleophilic C–H stretching vibration, indicating unsaturation
1653.04	C=O of carboxylic acid and derivatives
1743.89 (Figure 3(a))	C=O of ester group
1800–1500	Characteristic for proteins
1600–1500	Amide II bands, due to N–H stretching vibration
1200–900	C–O, C–C, C–O–C, C–O–P stretching vibrations of polysaccharides; CH3, CH2 rocking mode of vibrations
3100–2800	Presence of lipid
2922.77 (Figure 3(a)), 2924.50 (Figure 3(b))	CH2 asymmetric stretching in lipid
2851.32 (Figure 3(a)), 2853.23 (Figure 3(b))	CH2 symmetric stretching in lipid