Table 3: Lipid content at various pretreatment processes of N. oculata under nitrogen rich and nitrogen starved conditions.

Various pretreatmentPretreatment time (min/h)Normal growthNitrogen depleted growth

Control 26.43 40.52

Acid1 h31.2348.38
2 h33.1854.26
3 h33.1854.26

Enzyme8 h29.3845.28
10 h30.6248.52
12 h 32.74 51.68

Ultrasonication5 min28.1444.16
10 min29.3245.24
15 min30.1245.77

Autoclave10 min27.6842.39
20 min28.0643.38
30 min28.8143.90

Microwave oven5 min26.5141.28
10 min22.8835.36
15 min16.7923.21

40% NaCl24 h26.4540.54
48 h26.4940.56
72 h26.4940.56