Effect of Various Pretreatment for Extracting Intracellular Lipid from Nannochloropsis oculata under Nitrogen Replete and Depleted Conditions

Table 3

Lipid content at various pretreatment processes of N. oculata under nitrogen rich and nitrogen starved conditions.

Various pretreatment	Pretreatment time (min/h)	Normal growth	Nitrogen depleted growth
Control		26.43	40.52
Acid	1 h	31.23	48.38
	2 h	33.18	54.26
	3 h	33.18	54.26
Enzyme	8 h	29.38	45.28
	10 h	30.62	48.52
	12 h	32.74	51.68
Ultrasonication	5 min	28.14	44.16
	10 min	29.32	45.24
	15 min	30.12	45.77
Autoclave	10 min	27.68	42.39
	20 min	28.06	43.38
	30 min	28.81	43.90
Microwave oven	5 min	26.51	41.28
	10 min	22.88	35.36
	15 min	16.79	23.21
40% NaCl	24 h	26.45	40.54
	48 h	26.49	40.56
	72 h	26.49	40.56