Downregulation of RelA (p65) by Rapamycin Inhibits Murine Adipocyte Differentiation and Reduces Fat Mass of C57BL/6J Mice despite High Fat Diet
(a) RAPA inhibited the expression of p65/RelA and mTOR during adipocyte differentiation in a dose-dependent manner. Increasing amounts of RAPA were added to muADSC cell culture groups to determine dose dependency. After 6 days posttreatment, total proteins were extracted and Western blot analysis was performed with specific antibodies to RelA, RelB, c-Rel, and mTOR. The antibody to -actin was used for loading control. (b) RAPA represses the expression of adipogenic marker genes such as PPAR-gamma, C/EBP-, and aP2 during adipocyte differentiation. 10 nM RAPA was added into muADSC cell cultures. After 6 days posttreatment, total proteins were extracted and Western blot analysis was performed with specific antibodies to PPAR-gamma, C/EBP, and aP2. Expression of -actin was used for loading control. The results are representative of three independent experiments.
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