Table of Contents
ISRN Spectroscopy
Volume 2014 (2014), Article ID 541970, 7 pages
http://dx.doi.org/10.1155/2014/541970
Research Article

Novel Spectrophotometric Methods for the Determination of Selegiline Hydrochloride in Bulk and Its Pharmaceutical Preparation

Department of Post-Graduate Studies and Research in Chemistry, Mangalore University, Mangalagangothri 574199, India

Received 27 November 2013; Accepted 18 January 2014; Published 23 February 2014

Academic Editors: Y. C. Ling and T. Strunskus

Copyright © 2014 Kumble Divya and Badiadka Narayana. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple and highly selective spectrophotometric method has been developed for the determination of selegiline hydrochloride in bulk and formulations. Method A is based on the oxidation of 3-methyl-2-benzothiazolinone hydrazone in the presence of ceric ammonium sulphate, followed by its coupling reaction with drug to form a colored product having of 629 nm. Method B is based on the coupling reaction of drug with 4-aminoantipyrine to give a new ligand that reacts with copper(II) to give intense bluish red colored chelate which is measured at 539 nm. Beer’s law is obeyed in the range of 10.00–85.00 μg mL−1 with molar absorptivity of for method A and 20.00–120.00 μg mL−1 with molar absorptivity of for method B. The optimum reaction condition and the analytical parameters are evaluated. The results obtained indicate that the methods are free from interference of the ingredients; thus they are successfully applied to pharmaceutical formulations.