Table of Contents
ISRN Biomarkers
Volume 2014, Article ID 562587, 5 pages
Clinical Study

Evaluation of CD25+CD4+ Regulatory T-Lymphocyte Subpopulations in Coronary Artery Diseases Patients

1Department of Biochemistry, Silesian Medical University, Ul. Jordana 19, 41-808 Zabrze, Poland
2Department of Cardiology, Silesian Medical University, 41-808 Zabrze, Poland
3Department of Immunology, Silesian Medical University, 41-808 Zabrze, Poland

Received 9 December 2013; Accepted 15 January 2014; Published 4 March 2014

Academic Editors: H.-L. Chan and N. Lisitza

Copyright © 2014 Ewa Romuk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. The development of atherosclerosis may be associated with a deficiency in the regulatory T-cells, which should serve a protective function and inhibit the accumulation of lymphocytes and macrophages. The aim of this study was the analysis of the T-lymphocyte subpopulations, particularly CD4+CD25+ regulatory T-cells in patients with different form of coronary artery disease. Materials and Methods. In the study 30 patients with stable coronary heart disease and 30 patients with unstable coronary heart disease take part. Lymphocytes subpopulations were measured with flow cytometry technique. The analysis of the treated cells parameters was performed with the use of CellQuest program. Results. We have observed statistically significant increase in activated lymphocytes subpopulations in patients with unstable coronary artery disease in comparison to stable group and significant decrease in CD25+, CD25/CD3+, and CD25/CD4+ subpopulations in unstable patients comparing to stable patients group. Conclusions. A strong interest in regulatory lymphocytes is due to their possible therapeutic use as a factor in modifying the immune response in various diseases. Questions regarding the role of regulatory T-cells in the development of atherosclerosis remain unclear. Mechanisms of the regulatory T-cells impact on suppression of atherosclerosis need more experiments to be done.