Table of Contents
ISRN Inflammation
Volume 2014, Article ID 563628, 7 pages
Research Article

Crotalus durissus collilineatus Venom Induces TNF-α and IL-10 Production in Human Peripheral Blood Mononuclear Cells

1Departamento de Biomedicina e Farmácia, Pontifícia Universidade Católica de Goiás, 74605-140 Goiania, GO, Brazil
2Centro de Estudos e Pesquisas Biológicas (CEPB), Pontifícia Universidade Católica de Goiás, 74605-010 Goiania, GO, Brazil
3Laboratório de Imunogenética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil
4Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, 74605-050 Goiania, GO, Brazil

Received 9 October 2013; Accepted 2 December 2013; Published 19 January 2014

Academic Editors: A. El-Shazly, U. Musabak, T. Oshima, and T. Pessi

Copyright © 2014 Camila Bastos Ribeiro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Snake venom has been the subject of numerous studies in an attempt to find properties and biological effects that may be beneficial to man. In this study we evaluated in vitro the effects of Crotalus durissus terrificus (Cdt) and Crotalus durissus collilineatus (Cdc) venom in human peripheral blood mononuclear cells (PBMCs). At 24 h, a significant decrease of viable cells was observed in cells stimulated with the Cdc venom at 0.0005 mg/mL and 0.005 mg/mL compared to the negative control. At 48 h, a significant decrease of viable cells was observed only in cells stimulated with Cdc venom at 0.005 mg/mL. A significant increase of TNF-α and IL-10 was detected 48 hours after culture of PBMC with Cdc, but not with Cdt venom. The expression of CD69 and PD1 (programmed death-1), activation and regulatory cell markers, on CD8+ and CD8− T cells did not change in the presence of Cdt and Cdc venom. Our results suggest the presence of proinflammatory and anti-inflammatory components in the Cdc venom. Further analysis should be done to identify those Cdc venom components as it has been done for the Cdt venom by other authors, indicating that modulatory components are found in the venom of different species of Crotalus snakes.