Development and Validation of HPTLC Method for Simultaneous Estimation of Diosgenin and Quercetin in Fenugreek Seeds (<i>Trigonella  foenum-graceum</i>)

<table>Images of HPTLC separation of extracts of samples of fenugreek seeds. Tracks 1 and 13 represent diosgenin standard (500 ng per band) and 3–12 represent diosgenin from IL1, IL2, and IL3 seed cultivars. Tracks 14 and 26 represent quercetin standard (500 ng per band) and 15–24 represent quercetin from IL1, IL2, and IL3 seed cultivars. The mobile phase was Toluene-ethyl acetate formic acid (5 : 4 : 1,<i> v</i>/<i>v</i>/<i>v</i>).</table>

International Scholarly Research Notices

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Figure 2

Figure 2: Development and Validation of HPTLC Method for Simultaneous Estimation of Diosgenin and Quercetin in Fenugreek Seeds (<i>Trigonella  foenum-graceum</i>) 

Figure 2 | Development and Validation of HPTLC Method for Simultaneous Estimation of Diosgenin and Quercetin in Fenugreek Seeds (Trigonella  foenum-graceum) 